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Preventive Medicine 2018 Journal of Preventive Medicine
ISSN: 2572-5483 Page 59
July 16-17, 2018
London, UK
9th Edition of International Conference on
Preventive Medicine
& Public Health
Objective: Increasing evidence suggests that long non-coding
RNAs (lncRNAs) are involved in a variety of physiological and
pathophysiological processes of cancer, but whether lncRNAs
can serve as an important role in acute leukemia is still unclear.
This study was designed to investigate the effect of LncRNA-
ENST00000414355 in regulating cell proliferation and apoptosis
in acute myeloid leukemia.
Methods: HL-60，K562，THP-1 and U937 cells were transfected
with si-ENST00000414355 and si-NC, respectively, MTT assay
was performed to determine the proliferation, cell apoptosis
was assayed by flow cytometry using Annexin V-FITC/PI. Bone
marrow (BM) samples were obtained from 10 AML patients and
five healthy volunteers in Guangzhou First People’s Hospital. The
LncRNA-ENST00000414355 expression of the BM samples was
detected using qPCR.
Results: We found that the expression of LncRNA-
ENST00000414355 in AML cells was significantly knock-down
after 72 h treated with si-ENST00000414355. Down regulation of
LncRNA-ENST00000414355 in AML cell lines HL-60，K562，THP-1
and U937 inhibited cell proliferation, and induced cell apoptosis.
LncRNA-ENST00000414355 expression was markedly up-
regulated in AML patients’ bone marrow samples comparing with
the healthy volunteers’ (P < 0.01).
Conclusions: Results indicated that LncRNA-ENST00000414355
level up-regulated in AML and regulates cell proliferation and
apoptosis.
LncRNA-ENST00000414355 regulates cell proliferation and
apoptosis in acute myeloid leukemia
Caixia Wang1, Zhiheng Zhou2, Huanyu Shen 2, Baoxin Chen2,Qiulan Li , Shunqing Wang1
1Guangzhou First People’s Hospital, China
2Guangzhou Medical University, China
J Prev Med 2018, Volume 3
DOI: 10.21767/2572-5483-C1-003