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Pharmacognosy 2019
March 11-12, 2019
London, UK
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 29
Pharmacognosy and
Medicinal Plants
7
th
Edition of International Conference on
Cytotoxic activity of the root extracts of
Petroedmondia
syriaca (Boiss.) Tamamsch
Demet Akalgan
Girne American University, Cyprus
T
heisolatedandpurified12coumarinsfromtherootsof
the plant were identified as 3’-isobutyryloxymarmesin
acetate, 3′-α-angeloyloxydeltoin, smyrnioridin, deltoin,
marmesin acetate, scoparone, bergapten, psoralen,
colladonin, 14’- Acetoxybadrakemin, marmesin and 14’-
hydroxybadrakemin using their physical and spectral
characteristics, three compounds of them were isolated
for the first time in this study. The extracts (hexane,
CH
2
Cl
2
etc.) were evaluated for cytotoxicity by the MTT
method using MCF-7 cell line. The cytotoxic activity of
the compounds was obtained and the coumarin mixture
were investigated and the cytotoxic activity was found
to be significant. This is the first report on the chemical
composition and cytotoxic activity of the roots
P. syriaca
.
The cytotoxic activity of the coumarin mixture was found
to be significant. The highest activity was found in the
petroleumether and dichloromethane extracts. In order to
identify their cytotoxic compounds, petroleum ether and
dichloromethane extracts were subjected to a series of
chromatographic separations. Structures of the purified
compounds were elucidated by spectroscopic methods,
chemical transformations and direct comparison with
the authentic standards and they were available as
scoparone (1), psoralen (2), bergapten (3), marmesin
(4), marmesinacetate (5), deltoin (6), smyrnioridin (7),
4’-acetyl-3’- isobutyroyloxymarmesin (8), colladonin (9)
and 14- acetoxybadrakemin (10). Petroleum ether and
dichloromethane extracts of
Petroedmondia syriaca
(Boiss.) Tamamsch showed cytotoxic activity on MCF-7
cell lines at 25 μg/mL and higher concentrations (Figures
1&2). This is the first report on the chemical composition
and cytotoxic activity of the roots of
P. syriaca
. The
cytotoxic activity testing of the isolated compounds is
currently in progress. Cytotoxic activity of colladonin has
recently been reported.
Figure 1:
Figure 2: Cytotoxic activity of the petroleum ether extract
of
P. syriaca
Figure 3: Cytotoxic activity of the dichloromethane extract
of
P. syriaca
Demet Akalgan, Am J Ethnomed 2019, Volume 6
DOI: 10.21767/2348-9502-C1-008