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Pharmacognosy 2018 American Journal of Ethnomedicine
ISSN: 2348-9502 Page 62
April 16-17, 2018
Amsterdam, Netherlands
6th Edition of International Conference on
Pharmacognosy and
Medicinal Plants
T he anticancer activity of volatile isolates from Allium
commutatumGuss., Allium sativum L. (Amaryllidaceae family),
and Lepidium graminifolium L. ssp. graminifolium (Brassicaceae
family), analyzed by GC-MS, as well as the pure sulfur volatiles,
were evaluated against two human tumor cell lines: glioblastoma
cell line LN-229 and bladder cancer cell line UM-UC-3, using
MTT assay. The major sulfur volatiles found in A. commutatum
hydrodistillate from flower and A. sativum bulb originated from
S-alk(en)yl cysteine sulfoxide degradation. The most abundant
sulfur volatile in A. commutatum isolate was dipropyl trisulfide,
and in A. sativum diallyl disulfide. A. commutatum distillate
showed veryweak cytotoxic effect on both cancer cell lines except
at incubation time of 72 h on LN229 cell line (IC50 6.364 µg/mL),
while dipropyl trisulfide showed much stronger cytotoxic effect:
with IC50 12.34 and 10.35 µg/mL, and IC50 22.19 and 8.434 µg/mL
for UM-UC-3 and LN229 cell line, respectively during incubation
time of 48 and 72 h. Both A. sativum extract and distillate showed
strong time and concentration-dependent cytotoxic activity on
both cancer cell lines, with the best results at incubation time of
48 and 72 h. A. sativum extract had IC50 14.49 and 12.48 µg/mL,
and 40.84 and 10.41 µg/mL for UM-UC-3, and for LN229 cell lines,
respectively, during incubation time of 48 and 72 h. A. sativum
distillate showed very similar results: IC50 20.86 and 14.13 µg/mL,
and 17.41 and 12.01 µg/mL for UM-UC-3, and for LN229 cell line,
respectively. As expected, an active compound from A. sativum
diallyl disulfide showed very strong anticancerogenic potential
with IC50 22.3 and 19.07µg/mL, and 44.69 and 8.85µg/mL for UM-
UC-3, and LN229 cell line, respectively. L. graminifolium extract
and distillate sulfur volatiles originated from glucosinolates
degradation i.e. 3-methoxybenzyl isothiocyanate, and benzyl
isothiocyanate. They didn’t show strong cytotoxic activity on
UM-UC-2 cell lines. While there was effect on LN229 cell line of
distillate at incubation time of 48 hours (IC 50 53.92 µg/mL) and
of extract at incubation time of 48 and 72 hours (IC50 30.71 and
54.37 µg/mL, respectively). On the contrary, benzyl isothiocyanate
showed much stronger cytotoxic effect: with IC50 13.16 and 12.3
µg/mL, and IC50 6.48 and 12.29 µg/mL for UM-UC-3, and LN229
cell line, respectively during incubation time of 48 and 72 h.
Anticancer activity of Allium commutatum, Allium sativum, and
Lepidium graminifolium on human cancer cell lines
Vedrana Čikeš Čulić , Maja Katičić, Ana Polunić, Mirko Ruščić, Petra Brzović, Azra
Đulović and Ivica Blažević
University of Split, Croatia
Vedrana Čikeš Čulić et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006