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t h
E u r o p e a n C o n g r e s s o n
Obesity and
Eating Disorder
Obesity 2018
Journal of Obesity & Eating Disorders
ISSN 2471-8203
A p r i l 1 2 - 1 3 , 2 0 1 8
Am s t e r d a m , N e t h e r l a n d s
Page 56
Background:
Molecular mechanisms of most anti-obesity
drugs are remained to be clear. MicroRNAs that are non-
coding RNA molecules supposed to regulate biological
processes concomitant to obesity and have attracted a lot
of attention in scientific communities. miR-27a and miR-
143 expression levels in obese and non-obese rats during
weight changes and L-carnitine (LC) effects on them was
investigated in this study.
Material & Methods:
In the present study, 12 male Wistar
rats were randomly divided into normal-fat diet (NFD) and
high-fat diet (HFD) groups to develop obesity. After 8 weeks,
rats were weighted and half of diet induced obese rats were
randomly selected to receive 200 mg LC kg-1 body weight for
4 weeks. At the end, epididymal fat was isolated to investigate
expression level of microRNAs by real-time PCR.
Results:
After 12 weeks, HFD in comparison with NFD caused
significant decrease and increase in expression levels of miR-
27a and miR-143 respectively. These changes were modified
in groups which had received LC in a 4-weeks period.
Furthermore, rats in this group gained less weight.
Main Conclusions:
Findings of this study suggest that the
changes of microRNAs expression probably play a role in
pathogenesis of obesity. They might be modulated by means
of dietary agents and supplements and modify weight gain
trend.
maryam.nazary1@yahoo.comInfluence of L-carnitine on the expression level of
adipose tissue miRNAs related to weight changes in
obese rats
Maryam Nazari, Majid Karandish and Alihossein Saberi
Ahvaz Jundishapur University of Medical Sciences, Iran
J Obes Eat Disord 2018, Volume: 4
DOI: 10.21767/2471-8203-C1-009