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Page 48
Volume 05
Journal of Clinical Nutrition & Dietetics
ISSN: 2472-1921
JOINT EVENT
June 17-18, 2019 London, UK
Nutrition World 2019
Euro Obesity 2019
June 17-18, 2019
&
26
th
World Nutrition Congress
15
th
Euro Obesity and Endocrinology Congress
Stability of novel probiotics in fruit juices
Paramaporn Kerdsup
1
, Malai Taweechotipatr
1
and
Kittipong Asatarakul
2
1
Srinakharinwirot University, Thailand
2
Chulalongkorn University, Thailand
Statement of the Problem:
New probiotic products are globally growing and most companies attempt to own their
specific strain. There were many probiotic strains isolated from several sources in Thailand. The use of these isolated
strains to develop new alternative fruit-base product was a challenge pushing the novel probiotic to the commercial
level.
Aim:
This research aims to investigate the viability in fruit juices of three novel probiotic strains,
Lactobacillus
paracasei
MSMC39-1,
Lactobacillus casei
MSMC39-3, and
Lactobacillus salivarius
MSMC120-2, which were isolated
in Thailand. From the existing research, MSMC39-1 and MSMC39-3 were able to modulate immune system by
decrease the TNF-
α
production and MSMC120-2 showed the ability to induce IL-12 which involving antitumor
activity.
Methodology & Theoretical Orientation:
The free probiotic cells (8 log CFU/ml) were inoculated into orange and
guava juices and stored at 4
0
C for 35 days. Encapsulation technique with alginate and chitosan was used to find the
possibility to enhance cell survival. The viability of the target strain was assessed through MRS agar plate count
method.
Findings:
Free
L. casei
MSMC39-3 cells showed the best stability in orange and guava juices. Encapsulation technique
could be used to increase viability of the cells significantly (p 0.05). The encapsulated
L. salivarius
MSMC120-2
showed the highest rate of survival in both fruit juice. The encapsulation had no effect on chemical and physical
properties of the juices such as pH, total soluble solid and total phenolic of the juices (p>0.0) during storage.
Conclusion & Significance:
Orange and guava juice could be used as carrier for alginate and chitosan encapsulated
L. salivarius
MSMC120-2. The storage time was up to 21 days at 4
0
C with no change in the juice chemical and
physical properties. This could be the new alternative fruit-base product containing antitumor probiotic that suit
Asian people.
paramapornk@g.swu.ac.thJ Clin Nutr Diet 2019, Volume 05