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Page 42
Volume 05
Journal of Clinical Nutrition & Dietetics
ISSN: 2472-1921
JOINT EVENT
June 17-18, 2019 London, UK
Nutrition World 2019
Euro Obesity 2019
June 17-18, 2019
&
26
th
World Nutrition Congress
15
th
Euro Obesity and Endocrinology Congress
Evaluating the effects of low molecular weight sodium alginate as an immunostimulator, also as a novel
prebiotic combined with Bactocell® (a probiotic) on humoral and mucosal immune responses of Asian
seabass
(Lates calcalifer)
juveniles
1
Ghasem Ashouri,
1
Nasrollah,
1
Mahboobi Soofiani,
1
Amir Mani
and
2
Seyed Hossein Hoseinifar
1
Isfahan University of Technology, Iran
2
University of Agricultural Sciences and Natural Resources, Iran
D
uring the past decade, aquaculture has been considered as one of the fastest-growing industries of food
production. The elevation of aquatic animal production has been obtained by increasing the production per
unit of area which per se results from intensification. However, the elevation of stocking density in aquaculture
practices increases the stress level in the cultured organism and the risk of diseases. Following the 2006 EU ban on
antimicrobial growth promoters, to reduce use of antibiotics chemical compounds in agri-business products have
arisen. Therefore, application of bioactive substances, such as probiotics and prebiotics as an alternative to antibiotics
has been considered as environmentally friendly tool for aquaculture. Interest in the use of prebiotics and probiotics
in aquaculture increased considerably over the past several years due to the beneficial effects reported for these diet
additives in humans and terrestrial animals, including enhanced production efficiency, increased nutrient utilization,
.and improved disease resistance. Using immune stimulants as bio-friendly and environmentally safe agents is an
alternative approach to combat diseases in aquaculture. Therefore, present study performed to evaluate the potential
effect of individual or combined administration of dietary low molecular weight sodium alginate (LMWSA) and
Bactocell® on haematological, humoral and skin mucosal immune responses of
L. calcarifer
juveniles.
Methodology and Theoretical Orientation:
In this study six weeks of feeding trial was conducted to examine the
effect of dietary administration of low molecular weight sodium alginate (LMWSA) and
Pediococcus acidilactici
MA
18/5M (PA) on growth performance, digestive enzymes, and intestinal histology of
Lates calcarifer
juveniles. Fish
(12.0 ± 0.2 g ) were fed experimental diets as follows: Control (diet 1, basal diet), 5 g kg-1 LMWSA (diet 2), 10 g kg-1
LMWSA (diet 3), 0.9 × 107 CFU g-1 PA (diet 4), 5 g kg-1 LMWSA + 0.9 × 107 CFU g-1 PA (Diet 5), and 10 g kg-1
LMWSA + 0.9 × 107 CFU g-1 PA (Diet 6). At the end of the trial, blood samples from the caudal vein and skin mucus
were collected for evaluation of immunological parameters.
Findings:
Results indicated a significant (P < 0.05) increase in innate immune parameters including serum lysozyme,
bactericidal, hemolytic and respiratory burst activities as well as mucosal immune responses including lysozyme
and bactericidal activities when diet was supplemented with immunostimulants. Moreover, the combined effects
of LMWSA with Bactocell® resulted in more pronounced immunological responses compared to the control and
singular administration. Red and white blood cell counts significantly increased with either singular or combined
administration of LMWSA and Bactocell® compared with the control group (P < 0.05).
Conclusion and Significance:
These results indicated that combined administration of LMWSA and Bactocell® can
be considered as a beneficial feed additive and immunostimulant in
L. calcarifer
juveniles.
g.ashouri@na.iut.ac.irJ Clin Nutr Diet 2019, Volume 05