nanomat-2018-posters-accepted-abstracts

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NanoMat 2018 Nano Research & Applications
ISSN: 2471-9838 Page 78
April 26-27, 2018
Rome, Italy
17th Edition of International Conference on
Emerging Trends in
Materials Science and
Nanotechnology
Chitosan (CS) is recognized for its excellent performance
to interact with polyanionic macromolecules (such as
gellan gum, GG) to enable the production of physical hydrogels
(polyelectrolyte complexes; PECs). CS was associated with GG,
β -cyclodextrin (β-CD) and curcumin (CU) to create PECs for
wound healing purpose. CS/GG/ β-CD/CU hydrogels containing
large contents of poreswere produced by the first time, controlling
the pHs of the CS solutions and CS:GG/β-CD/CU weight ratios.
Stable PECs matrices were created using a suitable level of CU
(23mg) at GG/β-CD solutions in a 2/1 rate at pH 6.0. The CU could
organize the polymeric chains in the hydrogel networks, allowing
the formation of materials with well-defined pore structures.
After neutralization step, the hydrogels loaded with CU displayed
self-assembling behaviors. SEM images of the CS/GG/β -CD/CU
hydrogels before and after neutralization (performed in ultrapure
water) confirmed the reorganization of polymer chains. The self-
assembling capacity was also confirmed by thermal analysis.
Throughout the neutralization step, the CU was not released from
the PEC matrix. So, the β-CD/CU complex can stabilize the PECs
arrangements, permitting the development of a stable support
matrix (scaffold) to enhance the adhesion and proliferation of
human adipose-derived stem cells (ADSC cells). Scaffolds used
for wound healing and tissue engineering purposes should
similarly promote cell adhesion and provide mechanical and
chemical cues that can guide cell shape, cell migration, and
other relevant cellular activities. In this facet, CS/GG/β-CD/CU
hydrogel significantly promotes ADSC attachment, adhesion,
and proliferation. The number of adhered cells on the PEC
surface significantly increased throughout seven days (p<0.05).
Fluorescence and SEM images confirmed these statements.
Chitosan/gellan gum/ β-cyclodextrin/curcumin hydrogel as
a support matrix for enhancing adhesion and proliferation of
human adipose-derived stem cells
Alessandro F Martins1, Ariel C de Oliveira1, Bruno H Vilsinski1 and Matt J Kipper2
1Federal University of Technology, Brazil
2Colorado State University, USA
Nano Res Appl, Volume:4
DOI: 10.21767/2471-9838-C1-009