nanobiotechnology-2018-scientifictracks

Nanobiotechnology 2018

Page 24

Nano Research & Applications

ISSN: 2471-9838

E u r o S c i C o n C o n f e r e n c e o n

Nanotech & Nanobiotechnology

J u l y 1 2 - 1 3 , 2 0 1 8

P a r i s , F r a n c e

Optical nano-thermometers have been well developed to measure the

temperature distribution in live cells. Nano-sized indicators such as proteins,

organic dyes, quantum dots, polymer particles and nano-diamond, are

injected into live cells, and the change in intensity, peak position or lifetime

of luminescence spectra for the nano-indicators are used to reveal the

change of local temperatures. However, the results are remarkably affected

by local environment, e.g., pH value, cellular viscosity and ion concentration

in the cytosol, thus causing controversial arguments. Here, we report direct

measurement results for the temperatures of individual cultured cancer cells.

By using double-stabilized measurement system and array of micro-scale

thin-film thermocouples, we have reduced the system thermal noise down to

±5 mK and observed local increments in temperature for individual live cells

in the range of 30-280 mK. With further improvements, e.g., by using arrays

nano-scaled thermocouples, the current method is promising for real-time 2D

mapping for the local temperatures of a single cell.

Biography

Shengyong Xu received his BSc in Physics from the Peking

University in 1988 and PhD from Department of Physics,

National University of Singapore in 1999. He is currently a

professor with Department of Electronics, School of Electronics

Engineering and Computer Sciences, Peking University.

He has published more than 200 journal and conference

papers. His group currently works on the physics of electrical

communication among neuron cells and normal cells,

temperature sensing at the cell and sub-cell levels, as well as

electrostatic tweezers at micro-nano-scales.

Direct measurement of the local temperature increment for

individual live cells

Shengyong Xu

Peking University, China

Shengyong Xu, Nano Res Appl 2018, Volume 4

DOI: 10.21767/2471-9838-C2-011