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Nanobiotechnology 2018
Page 24
Nano Research & Applications
ISSN: 2471-9838
E u r o S c i C o n C o n f e r e n c e o n
Nanotech & Nanobiotechnology
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
Optical nano-thermometers have been well developed to measure the
temperature distribution in live cells. Nano-sized indicators such as proteins,
organic dyes, quantum dots, polymer particles and nano-diamond, are
injected into live cells, and the change in intensity, peak position or lifetime
of luminescence spectra for the nano-indicators are used to reveal the
change of local temperatures. However, the results are remarkably affected
by local environment, e.g., pH value, cellular viscosity and ion concentration
in the cytosol, thus causing controversial arguments. Here, we report direct
measurement results for the temperatures of individual cultured cancer cells.
By using double-stabilized measurement system and array of micro-scale
thin-film thermocouples, we have reduced the system thermal noise down to
±5 mK and observed local increments in temperature for individual live cells
in the range of 30-280 mK. With further improvements, e.g., by using arrays
nano-scaled thermocouples, the current method is promising for real-time 2D
mapping for the local temperatures of a single cell.
Biography
Shengyong Xu received his BSc in Physics from the Peking
University in 1988 and PhD from Department of Physics,
National University of Singapore in 1999. He is currently a
professor with Department of Electronics, School of Electronics
Engineering and Computer Sciences, Peking University.
He has published more than 200 journal and conference
papers. His group currently works on the physics of electrical
communication among neuron cells and normal cells,
temperature sensing at the cell and sub-cell levels, as well as
electrostatic tweezers at micro-nano-scales.
xusy@pku.edu.cnDirect measurement of the local temperature increment for
individual live cells
Shengyong Xu
Peking University, China
Shengyong Xu, Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C2-011