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Volume 4
Nano Research & Applications
ISSN: 2471-9838
Page 76
JOINT EVENT
August 16-18, 2018 | Dublin, Ireland
&
12
th
Edition of International Conference on
Nanopharmaceutics and Advanced Drug Delivery
25
th
Nano Congress for
Future Advancements
Nano Congress 2018
&
Nano Drug Delivery 2018
August 16-18, 2018
Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C3-015
Synthesis, functionalization and characterization of mesoporous silica nanoparticles for intravenous
doxorubicin delivery to cancer cells
Tichaona Nharingo
1
, Elumalai Rajasegarana
1
, Potlako Mafa
1
, Ashok Raichur
2
, Bhekie Mamba
3
and
Alex Kuvarega
3
1
UNISA, RSA
2
IISc, India
C
hemotherapy treatment of cancer has proved beneficial in increasing survival rate compared to radiation therapy, surgery,
and photodynamic therapy because the drugs are spread all over the body. However, conventional chemotherapy is toxic
to normal cells and requires very long treatment periods and cycles. Curative chemotherapy of human malignancies is also
hindered mainly by multi-drug resistance (MDR) and other chemo-resistance properties exhibited by the body. The purpose
of this study is to develop multifunctional drug carriers that can encapsulate, prevent premature drug release, and actively
and specifically release the drugs in a stimuli-responsive way. Mesoporous silica nanoparticles were synthesized by the sol-gel
method, functionalized by double bilayers of alginate and chitosan using the layer-by-layer technique and finally conjugated
with folic acid. Drug loading,
in vitro
drug release in phosphate buffered saline and acetate buffers,
in vitro
cytotoxicity assay,
intracellular uptake and drug internalization by living cells were investigated. Relatively high drug encapsulation efficiency and
loading capacity of 53% and 2.3% were achieved at pH 5.
In vitro
drug release confirmed absence of doxorubicin release by the
carrier at blood pH of 7.4 while an initial burst release was observed at acidic pH followed by a sustained drug release over a 36
hour period. MTT assay showed the biocompatibility of the drug carrier while confocal laser scanning microscopy proved the
hyper uptake and internalization of the multifunctional drug carrier. Exposure of free doxorubicin drug, drug loaded carrier
with and without folic acid on the surface to tumour cell line and normal HeLa cells, before and after folic acid blocking,
showed the efficient folic acid receptor assisted drug internalization by the tumour cells. The investigation offered a practical
route to the fabrication of biocompatible, pH responsive targeted active and sustained doxorubicin delivery to tumour cells.
nharingoticha@gmail.com