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Medchem & Toxicology 2018
Page 73
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
A n n u a l C o n g r e s s o n
Medicinal Chemistry,
Pharmacology and toxicology
J u l y 3 0 - 3 1 , 2 0 1 8
Am s t e r d a m , N e t h e r l a n d s
I
n this study, a sensitive high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method
established for simultaneously determining five main isosteroidal alkaloids (imperialine-3-β-Dglucoside (imperialine-G),
imperialine, peimine, hupehenine and yibeinoside A) in Siberian fritillary bulb, was applied to pharmacokinetic studies in rat
plasma. The plasma samples pretreated using liquid-liquid extraction with ethyl acetate were quantitated by multiple reaction
monitoring (MRM) via positive electrospray ionization (ESI) mode. Chromatographic separation was performed on an Intersil
ODS-2 column (5 μm, 4.6 ×150 mm) with a single fifteen minutes run using gradient elution. The mobile phrase consisted of
(A) 10 mM ammonium acetate (containing 0.1% of formic acid) and (B) methanol. Method validation results showed that the
developed method had good accuracy and precision over the corresponding linearity range for all the analytes. Besides, bench-
top, autosampler, freeze-thaw circulation and long-term storage stabilities met the acceptable limit. This study examined a
specific and robust method which was successfully applied to analyze rat plasma samples for pharmacokinetic study of five
isosteroidal alkalosids.
dyelimin@126.comSimultaneous determination of five isosteroidal
alkaloids in Siberian fritillary bulb in rat
plasma and its application in pharmacokinetic
study by HPLC-MS/MS
Min Liang, Jianzhong Wang, Yanping Liu and Liming Ye
West China School of Pharmacy, Sichuan University, China
J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C3-009