17 / 53
Medchem & Toxicology 2018
Page 61
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
A n n u a l C o n g r e s s o n
Medicinal Chemistry,
Pharmacology and toxicology
J u l y 3 0 - 3 1 , 2 0 1 8
Am s t e r d a m , N e t h e r l a n d s
S
ince the application of carbamates was found to be the best options as pesticides to control the pests thereby improving the
crop productivity. However, the indiscriminate application of these chemicals in farm practices has caused serious adverse
effects in many non-targets including mammalian systems. The present study has been undertaken to assess the carbofuran
induced alterations in the activity of acetyl choline esterase (AChE) in brain and serum and the time dependent recovery in the
presence of vitamin C or
C. limon
fruit extract. We have exposed the rats with two sub-lethal doses of carbofuran (20% and 40%
of LD
50
value i.e. 1.6 mg Kg
-1
and 3.2 mg Kg
-1
body weight) up to 72h at the interval of 24h. In another set, the carbofuran treated
animals were allowed to recover for next 48h. Under these experimental conditions, the activity profiles of AChE, a key target of
carbofuran, have been determined in brain and serum of rats. In control group, the level of AChE activity was 20 times higher in
brain as compared to that of serum. The carbofuran exposure resulted in sharp decrease in AChE activity in all these organs of
rats. The administration of vitamin C and
C. limon
fruit extract in carbofuran treated rats indicated significant recovery in enzyme
activity in brain, and serum, suggesting the ameliorative effects of vitamin C and
C. limon
fruit extract. However, after next 48h
from the carbofuran treatment, the activity profiles of AChE in rat’s brain, and serum indicated a trend of self-recovery in all the
experimental groups, albeit to varying extent. The results from present investigation reflected that the introduction of vitamin C
and
C. limon
fruit extract may offer protection from carbofuran induced toxicity in mammalian systems. The withdrawal from the
carbofuran exposure up to 48h may help in partial self recovery.
sharmabi@yahoo.comInhibition of mammalian acetylcholinesterase by
carbofuran and protection by vitamin C and Citrus
limon fruit extract
Sharma B, Jaiswal S K and Gupta V K
University of Allahabad, Uttar Pradesh, India
J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C3-009