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Infectious Diseases 2018 Journal of Prevention and Infection Control
ISSN: 2471-9668 Page 56
June 07-08, 2018
London, UK
8th Edition of International Conference on
Infectious Diseases
T he EPFL spin-off, Resistell, proposes revolutionary alternative
to culture based antibiogram, the current gold standard in
antibiotic susceptibility testing. The method has been developed
by the Laboratory of Physics of Living Matter (LPMV EPFL,
Lausanne, Switzerland) led by Prof. Giovanni Dietler and is based
on the detection of nano-scaled motion that corresponds to living
bacterial cells. Resistell is commercializing this technology and its
offering a diagnostic device. The cantilevers, also used in atomic
force microscopy, serve as sensors in the Resistell’s instrument.
Sensor deflection is measured by the laser-based system using
photodiodes as detectors. The bacteria are non-specifically
attached to the cantilevers, which are subsequently immersed it in
the custom-made fluid chamber. The dynamic fluctuations of the
sensor in the growth medium and after exposure to the antibiotic
are recorded and analyzed using custom-made software. The
oscillations of the sensor indicate whether the bacteria in the
applied sample are metabolically active. When the antibiotic
susceptible strains are exposed to the drug, they become non-
viable within minutes and the oscillations of the sensor return to
the level of abiotic sample. The variance of the signal acquired
in the growth medium and after exposure to antibiotic serves as
a marker of susceptibility or resistance. The pre-clinical data for
a wide range of fast and slow growing bacteria are available. The
MVP of a device is currently undergoing the clinical validation in
the Swiss hospital. Resistell allows for the real-time monitoring
of bacterial response to a drug, as well as the calculation of
minimal inhibitory concentrations (MIC) and minimal bactericidal
concentrations (MBC). Compared to the current gold standard
antibiogram, Resistell is able to identify the most effective
antibiotic in a matter of minutes rather than days. This means
effective treatment of the patient can start on day one.
Resistell: nano-motion based fast antibiogram
Danuta Cichocka1, Petar Stupar2, Wojciech Chomicki2, Sandor Kasas2 and Giovanni
Dietler2
1Resistell, Switzerland
2EPFL, Switzerland
J Prev Infect Cntrol 2018, Volume 4
DOI: 10.21767/2471-8084-C1-003