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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 39
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
A
doptive therapy with chimeric antigen receptor (CAR)-
modifiedTcellshas induced long-termremission inpatients
with CD19+ leukaemia but limited success in solid tumors. The
cellular mechanisms driving the immune responses mediated
by CART-cells still remain obscure. The aim of this study is
to directly observe immune synapse formation, tumor cell
killing and kinetics of CART cell activation by live cell imaging
to explore its potential against different types of cancer.
Second-generation CAR specific for CD19 antigen-expressing
CD28 co-stimulatory domain were co-cultured with CD19+
leukaemia Nalm6 and visualized in real time using time-lapse
microscopy. The infiltration and cytotoxicity of solid tumors by
both natural killer (NK) and T cells was studied against SK-N-As
neuroblastoma cell line spheroids to determine their synergy.
Live cell imaging of CART cell antitumor response showed
that CART cells not only induced direct cytolysis of tumor cells
but arrest cell division and migration. CART cells have also
demonstrated the indirect killing where they induce leukemic
cell apoptosis without stable conjugation. Thus non-cytolytic
mechanisms may play an important role in determining the
anti-leukemic activity of CART cells. Live cell imaging has
shown that both T and NK cells are capable of infiltrating
neuroblastoma spheroids. However, NK cells reduced the size
of spheroids more than T cells. Unlike T cells, NK cells were also
able to completely dissociate day 3 immature neuroblastoma
spheroids. Future studies will examine whether NK cells can
enhance the potency of GD2 ligand specific T cells targeting
solid tumors by dissociating the tumor capsule.
Biography
Kajal Chaudhry is a PhD student in the Graduate School of Biomedical En-
gineering at University of New South Wales. She completed her MTech in
Biochemical Engineering at Harcourt Butler Technical University, India. Her
research interests include Genetic Engineering, Live Cell Imaging and Im-
munology.
kajal15chaudhry@gmail.comLive imaging CAR19T therapy: immune response under a new light
Kajal Chaudhry
1
, Sylvie Shen
2, 3
, Liyuan Wang
1
, David Bishop
4, 5, 6
, Ken Mick-
lethwaite
4, 5, 6
, Alla Dolnikov
2, 3
and
Robert Nordon
1
1
University of New South Wales, Australia
2
Lowy Cancer Research Centre, Australia
3
Kids Cancer Centre-Sydney Children’s Hospital, Australia
4
Westmead Institute for Medical Research, Australia
5
Sydney Medical School-University of Sydney, Australia
6
Westmead Hospital, Australia
Kajal Chaudhry et al., J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-005