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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 38
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
H
uman chondrocyte sheets have attracted attention as
tissue-engineered cartilage for the treatment of articular
cartilage defects. However, the process of transferring the
human chondrocyte sheets to cartilage defects is complicated
because the cell sheets are thin and fragile. This study
investigated whether human chondrocyte sheets could adhere
to human cancellous bone and express cartilage-specific
markers. Human chondrocyte sheets were constructed using
osteoarthritic chondrocytes and temperature-responsive
culture plates.Monolayer and triple-layered chondrocyte sheets
were placed on the top of cancellous bones and cultured in
basal medium. The expressions of cartilage surface (lubricin)
and hypertrophic chondrocyte (collagen type X) markers in
the tissue structure were observed by immunofluorescence
staining. After one month, all the chondrocyte sheets were
firmly attached, with growth inside the cancellous bones, as
shown by fluorescence staining of the nuclei and stress fibers.
The cells also adhered and proliferated to reach confluence
on the tissue culture surface outside the cancellous bone,
indicating cell growth and viability. Moreover, the expressions
of lubricin and collagen type X were found in chondrocyte
cultures. Our results indicated that the human chondrocyte
sheets showed potential to adhere to cancellous bone with
expression of cartilage surface markers; although hypertrophic
markers were found in the cultures as we used osteoarthritic
chondrocytes. Attachment of human chondrocyte sheets to
cancellous bone could enhance the thickness and support
the structure of engineered cartilage tissue transferred to
the defective areas. This would be beneficial for researchers
to develop a protocol for the treatment of articular cartilage
defects.
Biography
Sopita Wongin is a posdoctoral fellow in Biological Engineering Program,
Faculty of Engineering, King Mongkut’s University of Technology Thonburi.
Sopita got a scholarship from the Royal Golden Julilee PhD Program and
completed her PhD at King Mongkut’s University of Technology Thonburi,
Thailand in 2017. During her PhD, she worked as a special research student
for a year in Laboratory of BioProcess Systems Engineering (BPSE), Depart-
ment of Biotechnology, Graduate School of Engineering, Osaka University,
Japan.
Her research interests lie in the area of cell sheet technology, tissue engi-
neering and advanced cell technology. Her recent publications include
Chon-
drogenesis and Hypertrophy in Response to Aggregate Behaviors of Human
Mesenchymal Stem Cells on a Dendrimer-Immobilized Surface (2017), Effect
of Cell Sheet Manipulation Techniques on The Expression of Collagen Type
II by Altering Stress Fiber Formation (2018) and Maintenance of the human
chondrocyte phenotype on a dendrimer-immobilized surface for chondrocyte
sheet engineering (2018).
sopitawongin@gmail.comConstruction of human chondrocyte sheets on cancellous bone
to treat cartilage defects
Sopita Wongin
1
, Saranatra Waikakul
2
, Pojchong Chotiyarnwong
2
and
Kwancha-
nok Viravaidya-Pasuwat
1
1
King Mongkut’s University of Technology, Thailand
2
Siriraj Hospital-Mahidol University, Thailand
Sopita Wongin et al., J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-005