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Page 33
Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
16
th
EuroSciCon Conference on
Immunology
M a r c h 1 1 - 1 2 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Immunology 2019
B
urkholderiamallei
is the causative organisms of glanders. Although now rare
in Western countries, organism has recently gained much interest because
of their unique potential as bioterrorism agent. The importance of design and
the use of rapid diagnostic techniques have been doubled considering its
widespread prevalence in the Middle East, especially in Iran, the disease cause
being imported from the neighboring countries and also very important use
of the disease as a biological weapon. The present study was performed on
100 serum samples of different equines. Malleination test was performed and
serums were analyzed using indirect immunofluorescence.
Burkholderia mallei
bacterium was cultured in glycerinated TSA and TSB media and was identified
by molecular techniques. After preparation and inactivation of the bacterial
suspension by heat, bacterial smear slides were prepared and were fixed by
different fixators. After the addition of tested horse serums and anti-horse
conjugated with fluorescein, slides were observed by immunofluorescence
microscopy. The malleination diagnostic method showed that there were 8
positive, 3 suspicious and 89 negative samples out of 100 serums. However,
when the indirect immunofluorescent was carried out a total of 89 and 11
samples were identified to be negative and positive, respectively. It was
concluded that indirect immunofluorescent method is quick and simple for the
diagnosis of glanders.
Biography
I'm head of Tuberculosis and Glanders Department of Razi
Vaccine and Serum Research Institute in Iran. I produce all type
of Tuberculin and also Mallein. Additionally our team produced
good absorbed ELISA kit for Paratuberculosis and also ELISA
kits for sheep and bovine Brucellosis too.
N.MOSAVARI@rvsri.ac.irComparison of malleination diagnostic test and
immunofluorescence in screening Glanders
Nader Mosavari
2
, Shima Jamooli
1
and Mahdi Assmar
3
1
Departemant of Microbiology, Faculty of Advance Sciences & Technology, Islamic Azad University
Pharmaceutical Sciences Branch, Iran
2
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension
Organization (AREEO), Iran
3
Pasteur Institute of Iran, Iran
Nader Mosavari et al., J Clin Immunol Allergy 2019, Volume:5
DOI: 10.21767/2471-304X-C1-008