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Page 31
Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
16
th
EuroSciCon Conference on
Immunology
M a r c h 1 1 - 1 2 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Immunology 2019
T
he immune response is so dynamic that its intensity and nature change
over time. The cells of the innate immune system signal through cytokines
that coordinate and shape the characteristics of adaptive immunity. Nucleotide
oligomerization domain (NOD)-like receptors (NLRs) is an intracellular receptor
family with 22 members in human and 34 members in mice. Although they
are mostly considered as the components of innate immunity, they have been
reported to set a gate between innate and adaptive immunity by countless
research. Of all the NLRs that have been discovered to date, AIM2, NLRP1,
NLRP3 and NLRC4 are known to form an inflammasome complex. A typical
inflammasome complex is a multiple protein complex that is composed of ASC
(Apoptosis associated speck-like protein containing a CARD) adaptor protein,
caspase-1 enzyme and the NLR protein. Following inflammasome activation,
IL1B and IL18 are cleaved by caspase-1 and released to the extracellular
environment. In this study, we examined how NLRC4, a member of NLR family,
regulates the immune responses. The preliminary data from in vivo suggested
a role for NLRC4 in eosinofilic functions during asthma and allergy. Based on
these preliminary data, we characterized NLRC4 further in vitro as the number
of eosinophils are significantly increased during asthma, allergic diseases
and parasitic infections in healthy individuals or wild type mice. In our case,
we observed a significant reduction in eosinophils of NLRC4 deficient mice.
Then we went ahead and studied NLRC4 in a human cell line of eosinophils
called EoL-1 for mechanistical studies. Even though, NLRC4 is known to form
an inflammasome complex, our preliminary data suggest no change in ASC
adaptor molecule, but induction of IL1B, therefore, NLRC4 in eosinofils may
not be interacting with ASC, but most likely be interacting with caspase-1 or
caspase-8, since we were able to induce IL1B after NLRC4 ligand treatment
in EoL-1 cells, an eosinofilic cell line. We plan to expand these experiments
to primary human eosinofils and investigate the eosinophilic functions in the
context of NLRC4. Our findings might be an important asset to treat the severe
clinical symptoms deriving from eosinophilia.
Biography
Ceren Ciraci has completed her PhD from Iowa State University
and Postdoctoral Studies from University of Iowa Inflammation
Program. She is currently serving as a Junior Faculty at Istanbul
Technical University.
ciracic@itu.edu.trRegulatory roles of NLRC4 in eosinophilic functions
Ceren Ciraci and Ilgin Akkaya
Istanbul Technical University, Turkey
Ceren Ciraci et al., J Clin Immunol Allergy 2019, Volume:5
DOI: 10.21767/2471-304X-C1-008