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Page 70
Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
16
th
EuroSciCon Conference on
Immunology
M a r c h 1 1 - 1 2 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Immunology 2019
Objective:
HIV-1/AIDS therapy remains concerned problems due to side effects of highly active antiretroviral therapy. Thus,
seeking of alternative strategies for HIV-1 inhibition is still needed.
Houttuynia cordata
Thunb (
H. cordata
) is a plant that possesses
several antimicrobial activities. This study aimed to investigate the cytotoxicity of
H. cordata
extract on CD4+ lymphocytes; the
anti-HIV-1 activity of the extract on CD4
+
lymphocytes and the effects of the extract on anti-HIV-1 enzymatic activities.
Methods:
Cytotoxicity of the extract was determined using MTT assay. To investigate anti-HIV-1 activity of
H. cordata
, C8166
cells were treated with the extract either before or after being exposed to HIV-1NPO3 and incubated for 72 h. The amounts of
p24 antigen were detected by ELISA. Anti-HIV-1 integrase and protease activities of both the water and ethanolic extracts of
H.
cordata
were determined using enzymatic assay.
Results:
No cytotoxicity of
H. cordata
extract up to 400 µg/ml was observed. The extract showed potent anti-HIV-1 activity with 69%
inhibition in pre-treated C8166 cells. However, HIV-1 replication was not significantly suppressed when the cells firstly exposed
to the virus and then treated with the extract.
H. cordata
water extract possessed inhibitory effect against HIV-1 integrase while
the ethanolic extract showed weakly inhibitory activity. Neither the water nor ethanolic extracts had anti-HIV-1 protease activity.
Conclusion:
This study demonstrated that
H. cordata
can inhibit HIV-1 at the early stages of infection without cytotoxicity. These
findings may lead to the development of a new effective anti-HIV-1 agent that is effective at the early stages of the infection.
wipawee.ni@psu.ac.thHouttuynia cordata shows potent anti-HIV-1
infection in vitro
Wipawee Nittayananta
1
, Surada Satthakarn
2
, Thippawan
Chuenchitra
3
, Supinya Tewtrakul
4
and Aornrutai Promsong
5
1
Thammasat University, Thailand
2
University of Phayao, Thailand
3
Armed Forces Research Institute of Medical Sciences, Thailand
4
Prince of Songkla University, Thailand
5
Princess of Naradhiwas University, Thailand
J Clin Immunol Allergy 2019, Volume:5
DOI: 10.21767/2471-304X-C1-009