epigenetics-2017-posters-accepted-abstracts

7 / 22

Page 95
Notes:
conferenceseries.com
Volume 3, Issue 4 J Clin Epigenet
ISSN: 2472-1158 Epigenetics 2017
November 06-08, 2017
EPIGENETICS & CHROMATIN
November 06-08, 2017 | Frankfurt, Germany
2nd International Congress on
Effect of cigarette smoking on circulating plasma miRNA in healthy individuals
Bengt Åke Andersson1,2, Shariel Sayardoust3 and Nongnit Laytragoon-Lewin1,2
1Jönköping University, Sweden
2Linköping University, Sweden
3Institute for Postgraduate Dental Education, Jönköping, Sweden
Introduction: Cigarette smoke contains toxic, carcinogenic, reactive and oxidant substances that causes DNA damage,
transform epithelial cells phenotype and genotype, induce inflammation and remodeling immune response. Micro RNAs
(miRNAs) are non-coding RNAs involved in gene expression in multicellular organisms at epigenetically level. MiRNA
influence cell cycle progression, inflammation and smoking related tumours. miRNA expression pattern is essential for correct
differentiation of immune cells.
Materials & Methods: A total of 140 healthy individuals, 39 current cigarette smokers and 101 individuals with no current or
previous history of tobacco use (non-tobacco users) were included in this investigation. A panel of 11 miRNA was chosen in
this study based on their documented associations in inflammation, immune response, oncogene regulation, tumor suppressor
genes and they were all expressed in analysed plasma. Extraction and quantification of miRNA from plasma, miRCURY LNA™
Universal microRNA PCR system (Exiqon, Denmark) were used.
Result & Discussion: Of the 11-analysed miRNA, only miR-21-5p showed statistically significant decreased levels in 39
smokers compared to 101 non-tobacco users. miR-21 has been described as an oncomir and increased levels of miR-21 have
been seen in several types of solid tumours and haematological malignancies. The epigenetic alterations of miRNA in smoking
related head and neck cancer will be evaluated in further studies.
Figure. Fold change between smokers and non-smokers on levels of miRNA . Adjustment was done for age and
gender. *Statistically significant levels between smokers and non-smokers.
Biography
Bengt Åke Anderson’s research projects focusing on diagnostic and prognostic biomarkers associated with the effect of cigarette smoking on human health, cancer
risk and clinical outcome of smoking related cancer.
Bengt Åke Andersson et al., J Clin Epigenet 2017, 3:4
DOI: 10.21767/2472-1158-C1-003