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conferenceseries
.com
Volume 3, Issue 4
J Clin Epigenet
ISSN: 2472-1158
Epigenetics 2017
November 06-08, 2017
EPIGENETICS & CHROMATIN
November 06-08, 2017 | Frankfurt, Germany
2
nd
International Congress on
11. Espada J et al. Regulation of SNAIL1 and E-cadherin function by DNMT1 in a DNA methylation-independent context.
Nucleic Acids Research. 39:9194, 2011
Figure 1. Potential new role for ROS in the epigenetic regulation of gene expression (A) One single dose of PT generates non lethal ROS levels (DCF detection) that
have physiological effects such as the stimulation of cell proliferation (B), but also could have other roles as the regulation of DNA methylation and gene expression,
through the oxidation of the 5mC in CGIs. (C) DNA Dot Blot analysis quantification shows that 5 days after PT, there is an increase in 5hmC levels, followed
by a decrease in 5hmC and 5mC levels 7 days after, compared to light control cells. (D) To test if the global reduction of 5mC levels is correlated to a change in
transcription levels, a stable transfection with a methylation reporter gene (that expresses GFP when unmethylated) was performed. From 5 to 7 days after PT, a
significant and gradual increase in GFP expression was detected, as shown in the images and in the graph. All images are representative of at least three independent
experiments. Scale bar: 10 and 40 µm, A and D, respectively. The mean ± SD values of at least three independent experiments are represented in each graph. **:
Significant, P ≤ 0.01. *: Significant, P ≤ 0,1.
Biography
María Inmaculada Calvo Sánchez holds a postdoc position in the Experimental Dermatology and Skin Biology Group of the IRYCIS, Ramón y Cajal University Hospital.
The research of this group is focused on cutaneus regenerative medicine and epigenetic changes induced by reactive oxygen species. Also she collaborates with
the Francisco de Vitoria University as an associate professor in the degree of Biotechnology and in the Master's Degree in Advanced Therapies and Biotechnology
Innovation, and as a coordinator of the practical part of the Masters mentioned above.
calvosanchezmaria@gmail.com