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Volume 3, Issue 2 (Suppl)
Trends in Green chem
ISSN: 2471-9889
Environmental & Green Chemistry 2017
July 24-26, 2017
Page 27
5
th
International Conference on
6
th
International Conference on
July 24-26, 2017 Rome, Italy
Environmental Chemistry and Engineering
Green Chemistry and Technology
&
Expanding the reaction space of aldolases using hydroxypyruvate as a nucleophilic substrate
Virgil Helaine
1,2,3,4
, V de Berardinis
2
, C Guérard-Hélaine
2,4
, E Darii
2
, K Bastard
2
, A Mariage
2
, J L Petit
2
, N Poupard
1,2
, I Sánchez-Moreno
1,2
, M Stam
2
, T Gefflaut
2,4
,
M Salanoubat
2
and
M Lemaire
2,4
1
Université Clermont Auvergne, France
2
National Center for Scientific Research (CNRS), France
3
Sigma Clermont, France
4
Institut de Chimie de Clermont-Ferrand, France
A
ldolases are key biocatalysts for stereoselective C–C bond formation allowing access to polyoxygenated chiral units through
direct, e cient, and sustainable synthetic processes. The aldol reaction involving unprotected hydroxypyruvate and an aldehyde
o ers access to valuable polyhydroxy-α-ketoacids. However, this undescribed aldolisation is highly challenging, especially regarding
stereoselectivity. This reaction was explored using biocatalysts, a collection of aldolases selected from biodiversity. Several enzymes
were found to produce the desired hexulosonic acids from hydroxypyruvate and D-glyceraldehyde with complementary stereo-
selectivities. One of them was selected for the proof of concept as a biocatalytic tool to prepare five (3S, 4S) aldol adducts through an
eco-friendly process.
Biography
Virgil Helaine has completed his PhD from Clermont-Ferrand University, France. He has left for Darmstadt (Germany) where he has joined Prof. W D Fessner’s
group. Since 2000, he is an Assistant Professor in the Institute for Chemistry of Clermont-Ferrand (France) and his field of interest is focused on biocatalysis
especially the development of tools for eco-compatible synthesis: discovery and study of new enzymes, and their orchestration in multienzymatic cascade
processes towards compounds of biological interests.
virgil.helaine@uca.frVirgil Helaine et al., Trends in Green chem, 3:2
DOI: 10.21767/2471-9889-C1-002