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Dental Practice 2019 Dentistry and Craniofacial Research
ISSN: 2576-392X Page 36
January 28-29, 2019
Barcelona, Spain
27th International Conference on
Dentistry and Dental Practice
Background: Periodontitis reported was more often found in
SLE patients than healthy controls, 54.3% higher than that of
patientswithnosystemicabnormalityof 28.2%and theestimate
was related to cytokine inflammatory because of the aging of
immune system. Expression of IL-2 and IL-10 associated with
immune aging as T cell CD28 and CD45RO, were associated
with differentiation, cytotoxic activity, signaling and apoptosis
of T cells were known as biomarker of immune aging.
Objectives: To analyze correlation between periodontitis
severity with disease activity, IL-2, IL-10, CD28, and CD45RO
expression in SLE patients.
Methods: Subjects were 45 patients with SLE (age 17-54
years; SLEDAI score 0-42) collected from Dr. Saiful Anwar
General Hospital, Malang Indonesia. Periodontitis severity was
measured using Periodontal Index (PI) criteria. Expression of
IL-2 and IL-10 using ELISA and CD28, CD45RO was examined
using flow cytometry.
Result: Clinical manifestations of periodontitis were bleeding
gum 88.3%, high calculus index 44.9%, periodontal pocket
73.8% and loose teeth 13.2% among patients. PI score patients
were 2.45±0.82. There was significantly positive correlation
between PI score and SLEDAI score (r=0.798; p=0.000),
with IL-2 (r=0.512; p=0.000), with IL-10 (r=0.720; p=0.000)
with CD28 expression (r=0.634; p=0.000), and with CD45RO
expression (r=0.354; p=0.020).
Discussion: CD28 expressed specific CD8+ cells and natural
killer cells during the latent period of chronic infection,
especially on SLE patient. Repeated and persistent stimulation
of the antigen leads to increased CD expression in CD8+ T. T
cell CD28+ CD8+ cells are more susceptible to activated cell-
induced cell death, stimulated by mitogen. The evidence shows
that this cell can be used as a SLE marker. Differentiated CD8+
T cells experience loss of CD28 expression and CD45RO re-
expression. This cell effector capacity is evidenced by its
high capacity to produce perforin, granzymes, IFN-γ, IL-2 and
IL-10. In periodontitis, tissue damage also results from the
production of various irregular and unregulated productions of
inflammatory mediators and destructive enzymes in response
to the presence of bacterial biofilms and the process of
periodontitis.
Conclusion: Our study showed that periodontitis were
associated with SLE disease activity and biomarker of immune
aging. Furthermore, SLEDAI index and these markers could be
predictor for periodontal condition, prognosis of periodontitis
and best treatment for periodontitis on SLE patient.
Periodontitis associated with disease activity,
immune aging and inflammatory cytokine of
systemic lupus erythematosus patients
Nanda Rachmad Putra Gofur, Nurdiana, Kusworini Handono and Handono Kalim
Brawijaya University, Indonesia
J Den Craniofac Res 2019, Volume 4
DOI: 10.21767/2576-392X-C1-015