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Dental Practice 2019 Dentistry and Craniofacial Research
ISSN: 2576-392X Page 38
January 28-29, 2019
Barcelona, Spain
27th International Conference on
Dentistry and Dental Practice
J Den Craniofac Res 2019, Volume 4
DOI: 10.21767/2576-392X-C1-015
Introduction: Clinical applications of bioactive materials
are increasing in biomedical tissue engineering. This study
was sought to assess the effect of calcium enriched mixture
(CEM) cement, biodentine, mineral trioxide aggregate (MTA),
octacalcium phosphate (OCP) and Atlantik on proliferation,
odontogenic/osteogenic differentiation and pro-inflammatory
cytokine production by human stem cells of the apical papilla
(SCAPs).
Methods: The SCAPs were cultured and exposed to CEM
cement, Biodentine, MTA, OCP, Atlantik and no biomaterial
(control group). Proliferation of SCAPs treated with different
biomaterials was evaluated using trypan blue exclusion test
and flow cytometry. Differentiation of cells was evaluated
using alkaline phosphatase (ALP) activity, alizarin red staining
and reverse transcription polymerase chain reaction (RT-PCR).
The expression of genes of pro-inflammatory cytokines was
also evaluated using RT-PCR.
Results: The SCAPs treated with 20 mg/mL CEM cement,
2 mg/mL Biodentine, 200 µg/mL OCP/MTA and 20 µg/mL
Atlantik showed significantly higher proliferation, increased
ALP activity, higher number of calcified nodules and up-
regulation of genes related to odontogenic/osteogenicmarkers
[including ALP, runt-related transcription factor 2 (RUNX2),
osterix (OSX), osteocalcin (OCN), bone sialoprotein (BSP) and
dentin sialophosphoprotein (DSPP)] compared to the control
group. The expression of pro-inflammatory cytokines namely
interleukin (IL)-1α, IL-1β, IL-6 and tumor necrosis factor
(TNF)-α significantly increased in all groups compared to the
control group. The highest expression on day seven belonged
to IL-1α and IL-1β in SCAPs treated with MTA, IL-6 in SCAPs
treated with CEM cement, and TNF-α in SCAPs treated with
Atlantik and OCP.
Conclusion: CEM cement, Biodentine, MTA, OCP and Atlantik
can induce odontogenic/osteogenic differentiation in SCAPs.
MTA had a greater potential to induce differentiation of SCAPs
to odontoblast like cells while OCP had higher potential to
induce differentiation of SCAPs to osteoblast-like cells.
Odonto/osteogenic differentiation and cytokine
production by human stem cells of the apical
papilla induced by biomaterials: A comparative
study
Eshagh Ali Saberi
Zahedan University of Medical Sciences and Health Services, Iran