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E u r o S c i C o n C o n f e r e n c e o n
Dental & Dental
Hygiene
Dental & Dental Hygiene 2018
Journal of Dental and Craniofacial Research
ISSN 2576-392X
M a r c h 2 6 - 2 7 , 2 0 1 8
E d i n b u r g h , S c o t l a n d
Page 71
Acrylamide, one of the major environmental public health
problems, results from its increased accumulation in
the process of cooking food materials. This study aimed to
demonstrate the light and electron microscopic structural
effects of acrylamide on the skeletal muscle fibres of adult
male albino rat tongue and to investigate the possible
protective effect of vitamin E co-administration. Thirty
adult male albino Sprague–Dawley rats were divided into 3
groups, each group included 10 rats. Group I (control), group
II which was subdivided into two equal subgroups: subgroup
IIa: included 5 rats that received acryl-amide orally once
daily for 20 days. Subgroup IIb: included 5 rats that received
acrylamide orally once daily for 40 days. Group III was also
subdivided into two equal subgroups: sub-group IIIa: included
5 rats that received acrylamide and vitamin E orally once
daily for 20 days. Subgroup IIIb: included 5 rats that received
acrylamide and vitamin E orally once daily for 40 days. At
the end of the experiment the tongue was dissected out for
histological and electronmicroscopic studies, another muscle
sample was homogenized and processed for biochemical
estimation of malondialdehyde (MDA) and total antioxidant
capacity (TAC). Light microscopic study of tongue skeletal
muscles in acrylamide exposed animals revealed abnormal
wavy course and splitting of the muscle fibres with fatty
infiltration in between. Moreover, pyknosis and remnants of
nuclei were detected. EM (electron microscopy) revealed
marked aggregation of mitochondria of different size and
The impact of vitamin E against acrylamide
induced toxicity on skeletal muscles of adult
male albino rat tongue: light and electron
microscopic study
Rasha H Al Serwi and Fatma M Ghoneim
Mansoura University, Egypt
shape with giant cells formation, and partial loss of myofilaments.
There was statistically significant increase in MDA and decrease
in TAC indicating oxidative stress in acrylamide administrated
groups (group II) than the control group which increased by
prolonged duration (subgroup IIb versus subgroup IIa, p<0.0001).
This oxidative stress could explain the histological changes in
tongue muscles of acrylamide exposed rats. Co-administration
of vitamin E with acrylamide ameliorated most of the above
mentioned histological changes in the animals used and signs of
improvement that became better with prolonged administration of
it (subgroup IIIb versus subgroup IIIa, p<0.0001) were detected.
It could be concluded that, chronic exposure to acrylamide might
lead to skeletal muscle damage in rat tongue which becomes worth
with prolonged duration of exposure. Acrylamide induced oxidative
stress is the implicated mechanism of such histological changes.
This toxic effect of acrylamide could be minimized when vitamin E
is given concomitantly with it by its antioxidant effect.
J Den Craniofac Res 2018, Volume: 3
DOI: 10.21767/2576-392X-C1-003