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E u r o S c i C o n C o n f e r e n c e o n
Dental & Dental
Hygiene
Dental & Dental Hygiene 2018
Journal of Dental and Craniofacial Research
ISSN 2576-392X
M a r c h 2 6 - 2 7 , 2 0 1 8
E d i n b u r g h , S c o t l a n d
Page 70
The effect of atorvastatin on glucocorticoid-
induced osteoporosis on periodontal bone loss
Paula Goes
1
, Luzia Hemínia de Sousa
2
, Eveline Linhares
2
, Joana Trycia Alexandre
2
,
Mario Roberto Lisboa
3
, Mirna Marques
2
, Helliada Vasconcelos
2
, Conceição Martins
3
and Gerly Anne de Castro Brito
3
1
Federal University of Ceará, School of Medicine, Brazil
2
Federal University of Ceará, Sobral, School of Medicine, Brazil
3
Federal University of Ceará, School of Medicine, Brazil
Background:
Atorvastatin (ATV) has shown pleiotropic effects
on bone tissue, and osteoporosis can aggravate periodontitis.
Thus, we assessed the effects of ATV on experimental
periodontitis (EP) of rats subjected to glucocorticoid-induced
osteoporosis (GIOP).
Methodology:
Male Wistar rats were divided into: Naïve, EP,
GIOP+EP and ATV groups. GIOP+EP and ATV received 7 mg/
kg of dexamethasone intramuscularly 1x/week for 5 weeks, the
others received Saline (SAL). EP, GIOP+EP and ATV were
submitted to EP by ligature around 2nd upper left molars for
11 days. ATV received 27 mg/kg of ATV orally and the others
SAL, 30 minutes before EP. Periodontium was analyzed
by macroscopy, micro-tomography and histopathology; by
immunohistochemical examination of RANKL, OPG, WNT10b,
DKK-1 and β-catenin and by ELISA analysis of myeloperoxidase
(MPO), TNF-α, IL-1β, -6, -8, and -10, reduced glutathione (GSH),
superoxide dismutase (SOD) and catalase (CAT). Leukogram
and liver and kidney enzymes and bone-specific alkaline
phosphatase (BALP) serum levels were performed.
Results:
ATV decreased bone loss, reduced MPO, TNF-α, IL-
1β, -6, and -8, and increased IL-10, GSH, SOD and CAT levels.
ATV reduced RANKL and DKK-1, increased OPG, WNT10b and
β-catenin expressions and BALP activity.
Conclusions:
ATV reduced inflammation, oxidative stress and
bone loss in rats with EP and GIOP, with participation of WNT
signaling pathway.
paulagpinheiro@yahoo.com.brJ Den Craniofac Res 2018, Volume: 3
DOI: 10.21767/2576-392X-C1-003