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Chemistry Education 2018 Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123 Page 50
August 27-28, 2018
Zurich, Switzerland
8th Edition of International Conference on
Chemistry Education
and Research
C arbon-based nanotechnology is a promising field of research
for its potential application in the fields of bio-sensors, health
care and biomedical sciences. Graphene nanosheets due to
their one atom thickness are the ideal candidate for trapping
the bacteria for transmission and scanning electron microscopy
imaging. In this paper, we report on the antibacterial activity of
reduced graphene oxide nanosheets synthesized by modified
Hummer’s method. MRGO nanosheets were characterized
using scanning electron microscopy (SEM), transmission
electron microscopy (TEM) and atomic force microscopy (AFM)
along with disc diffusion technique, which was employed to
evaluate anti-bacterial activity of nanosheets using S. aureus as
model microorganism. The cell viability of S. aureus decreased
significantlywhenitwasexposedtoMRGOnanosheetsasrevealed
in the spectrophotometric study of bacterial growth. A clear zone
of inhibition was also observed in the cultured plates treated with
MRGO, revealing its antibacterial property. AFM study revealed
that structural defects of MRGO were probably responsible for
membrane integrity disruption leading to bacterial cell death. To
elucidate the bactericidal activity, SEM and TEM studies were
carried out. Studies showed that the defects formed in the MRGO
synthesis is the main factor for the effective antibacterial activity.
Further molecular studies are required to completely reveal the
antibacterial property of MRGO nanosheets.
Biography
Ronojoy Sen Gupta, has completely his PhD degree in Physiology from
the Department of Physiology, University of Calcutta, Kolkata, India. He
has worked as Postdoctoral fellow in Molecular Endocrinology laboratory,
Hormone Research Center, Chonnam National University and Department
of Life Sciences, Gwangju Institute of Science and Technology, Gwangju,
South Korea. He has worked as faculty in Department of Biotechnology and
Bioinformatics, SMMTR, Sikkim Manipal University, India. At present he is
working as Director, Genobiotek, Kolkata, India. Alok Chattopadhyay, has
completed his PhD degree in Physiology from the Department of Physiol-
ogy, University of Calcutta, Kolkata, India. He is now working as Assistant
Professor, Department of Physiology, Harimohon Ghosh College, University
of Calcutta, India.
Investigation of the antibacterial activity of modified reduced
graphene oxide (MRGO) nanosheets against S. aureus
Ronojoy Sen Gupta1 and Alok Chattopadhyay2
1Genobiotek, India
2Harimohan Ghose College, India
Ronojoy Sen Gupta et al., J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C5-015