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Chemistry Education 2018
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
Page 70
August 27-28, 2018
Zurich, Switzerland
8
th
Edition of International Conference on
Chemistry Education
and Research
Aims:
To screen hexane, ethyl acetate and methanol fractions
of the methanol extract of Spathodea campanulata leaves for
secondarymetabolites, to isolate and to characterize constituents
of the ethyl acetate fraction using GC-MS and IR and to determine
the antioxidant activities of the three fractions.
Methodology:
Methanol extract of Spathodeacampanulata leaves
was obtained by cold extraction, and partitioned into hexane, ethyl
acetate and methanol fractions. Phytochemical screenings of the
fractions were carried out using standard procedures to identify
the class of constituents present in each of them. Ethyl acetate
fraction was subjected to column chromatographic separations
by gradient elution, and isolates were TMS (trimethylsilyl)
derivatized and characterized by GC-MS (gas chromatography-
mass spectrometry). Antioxidant content was also evaluated on
the three fractions using 2,2-diphenyl-picrylhydrazyl (DPPH) free
radical scavenging method. Percentage of inhibition and IC50
values were obtained for each fraction.
Results:
Phytochemical screenings revealed presence of
alkaloids, tannins, saponin, resins, phenol, cardiac glycosides,
steroids, flavonoids, anthraquinones and terpenoids in the three
fractions in varying concentrations. Alkaloids, resins, phenol
and cardiac glycosides were found to be intense in the three
fractions while phlobatannins was found to be absent in all
the three fractions. Three compounds isolated from the ethyl
acetate fraction were characterized based on MS and IR spectral
interpretations as palmitic acid, ethylamine and caffeic acid.
Percentage of inhibition of the three fractions indicates that they
have substantial antioxidant activity with the standards at high
concentration of 250 to 1000 μg/mL. The hexane fraction has the
highest antioxidant activity with an IC50 of 178.46 μg/mL when
compared to other fractions.
Conclusion:
This paper reports phytochemical constituents
and high antioxidant activity (at concentrations of 250 μg/ mL
and above) of the African tulip tree (Spathodea campanulata)
when compared to the standards. This has not been earlier
reported in literature, our results support its wide ethno-medicinal
applications.
emmanuel.ojah@yahoo.comPhytochemical and antioxidant assessments of three
fractions from methanol extract of Spathodea campanulata
Beauv. leaves
Ojah Emmanuel Onah
University of Ibadan, Nigeria
J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C5-015