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Chemistry Education 2018
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
Page 67
August 27-28, 2018
Zurich, Switzerland
8
th
Edition of International Conference on
Chemistry Education
and Research
I
n
situ
generated fluorescent gold nanoclusters (Au-NCs) are
used for bio-imaging of three human cancer cells, namely,
lung (A549), breast (MCF7), and colon (HCT116), by confocal
microscopy. The amount of Au-NCs in non-cancer cells (WI38 and
MCF10A) is 20–40 times less than those in the corresponding
cancer cells. The presence of a larger amount of glutathione
(GSH) capped Au-NCs in the cancer cell are ascribed to a higher
glutathione level in cancer cells. The Au-NCs exhibit fluorescence
maxima at 490–530 nm inside the cancer cells. The fluorescence
maxima and matrix-assisted laser desorption ionization (MALDI)
mass spectrometry suggest that the fluorescent Au-NCs consist
of GSH capped clusters with a core structure (Au8-13). Time-
resolved confocal microscopy indicates a nanosecond (1–3 ns)
lifetime of the Au-NCs inside the cells. This rule out the formation
of aggregated Au–thiolate complexes, which typically exhibit
microsecond (.1000 ns) lifetimes. Fluorescence correlation
spectroscopy (FCS) in live cells indicates that the size of the
Au-NCs is .1–2 nm. For in situ generation, we used a conjugate
consisting of a room-temperature ionic liquid (RTIL, [pmim][Br])
and HAuCl4. Cytotoxicity studies indicate that the conjugate,
[pmim][AuCl4], is non-toxic for both cancer and non-cancer cells.
mdasifamin007@gmail.comCancer cell imaging using in
situ
generated gold nanoclusters
Md Asif Amin
1
and
Kankan Bhattacharyya
2
1
Balurghat Mahila Mahavidyalaya, India
2
Indian Institute of Science Education and Research, India
J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C5-015