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August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
Hypoglycemic mechanism of guava leaf extract: slow inactivation of protein tyrosine phosphatase 1B
(PTP1B)
Henry J Tsai
Asia University, Taiwan
G
uava leaf tea, has been used as a folk medicine
for treating hyperglycemic conditions in Asia and
Africa. The hypoglycemic efficacy of guava leaf has been
documented by many scientists in these regions, but the
hypoglycemic mechanism is poorly understood. Guava
leaves were extracted with methanol and the crude extract
was partitioned against hexane, ethyl acetate, and butanol
in sequence. The leftover in water is defined as the aqueous
partition. A second smaller batch was extracted with hot
water directly. Our study confirmed the hypoglycemic efficacy
on healthy mice and found the most effective molecules
reside in the aqueous partition which is also less cytotoxic
to Chinese hamster ovary cells when compared to other less
polar partitions. Therefore, the guava leaf tea can serve as
a functional hypoglycemic drink that is suitable for either
healthy or diabetic subjects. Coincidentally the aqueous
partition possesses a potent inhibitory effect on protein
tyrosine phosphatase 1b (PTP1B) enzymatic activity and this
PTP1B inhibition is through a slow oxidative inactivation on
the enzyme.
Speaker Biography
Henry J Tsai obtained his PhD in Biochemistry (1996) and MS in Nutritional Science
(1990) from the Michigan State University. He is currently an Associate Professor at the
Department of Health and Nutrition, Asia University, Taiwan.
e:
henrytsai@asia.edu.twHenry J Tsai, Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-002