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August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
alliedacademies
Ann Biol Sci, 2017
ISSN: 2348-1927
My laboratory discovered that some bovine antibodies
are the largest known to exist in a species. This is
because of generation of an exceptionally long CDR3H
(up to 61 amino acids) which is encoded by an unusually
long germline IGHD genes together with an unique
insertion of “A” nucleotide rich conserved short nucleotide
sequence at the IGHV-IGHD junction. The atypical CDR3H
provides a “knob and stalk” structure capable of creating
configurational diversity via variable intra-CDR3H disulfide
bridges within the knob. The knob is held by the solvent
exposed stalk formed by anti-parallel beta strands. I have
exploited these structural features of the bovine antibody for
the development of new therapeutics and vaccines. In this
context, I will provide insights into the structural optimization
of anti-viral bovine scFv to enhance their potency, apart
from discussing influence of framework residues on viral
neutralization function. I will provide ‘proof of concept’ for
developing new vaccines by antigenizing bovine antibody
with exceptionally long CDR3H that induce specific immune
response. First, I successfully developed functional bovine
scFv with an exceptionally long CDR3H followed by grafting
of a viral B-epitope into the CDR3H. The grafted B-epitope
in the exceptionally long CDR3H of bovine scFv sustained its
native configuration and induced desired specific antibody
response. Thus, antigenization of bovine scFv with an
exceptionally long CDR3H provides a novel approach to
developing new vaccines against infectious disease.
Speaker Biography
Azad K Kaushik has published two books Molecular Immunobiology of Self-Reactivity
(1992) and Comparative Immunoglobulin Genetics (2014) and over 87 research arti-
cles. He is on the editorial boards of several immunology journals and is a Consultant
to various international organizations. He was recognized as The Esther Z Greenberg
Honors Chair in Biomedical Research, and Visiting Professor, Oklahoma Medical Re-
search Foundation, USA, in 1998. He received BVSc&AH (Honors) in 1976 and MVSc
(1978) from the Faculty of Veterinary Science, Hisar, Haryana, India; followed by Doc-
teur es Science (DSc) in Immunology (1987) from the Pasteur Institute (University of
Paris VII), Paris, France. He has been teaching Immunology at the University of Guelph
since 1991.
e:
Azad K Kaushik
University of Guelph, Canada
Developing novel vaccines for neonatal immunization
Azad K Kaushik, Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-002