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American Journal of Computer Science and Information Technology
ISSN: 2349-3917
E u r o s c i c o n C o n f e r e n c e o n
3D Printing and Wireless
Technology
S e p t e m b e r 1 7 - 1 8 , 2 0 1 8
L i s b o n , P o r t u g a l
Wireless and Printing Technology 2018
B iodegradable stents (BRS) were introduced to overcome the limitations of
permanent stents, offering the potential to improve long-term patency rates
by providing support just long enough for the artery to heal itself. Ideally, BRS
shouldmeet four requirements: a) BRSmanufacturing process should be precise,
fast, and economic, b) BRS degradation should not be toxic to the body, c) BRS
degradation rate should match the recovery rate of vascular tissue, and d) BRS
should induce rapid endothelialization. While the second and third requirements
have been deeply studied, the first and last requirement had been overlooked.
This work presents a novel 3D printed BRS made of Polycaprolactone (PCL)
and Polylactic acid (PLA). Authors aim to study effects of different sterilization
methods over the stents properties and effects of stents material and geometry
over the cell proliferation. Three sterilization methods were applied such
as ethanol 70%, ultraviolet lamp and antibiotic sterilization. All treatments
were performed for 0.5h, 1h, 8h and 16h. Supplemented Dulbecco’s Modified
Eagle’s Medium (DMEM) with phenol red (pH indicator) and without penicillin/
streptomycin was added after different treatments. Remaining infection was
indicated by yellowing of the media and increased of media opacity. With
regards to ethanol treatment, all samples treated below 8 hours showed signs
of infection, while samples treated during 8 and 16 hours exhibited a complete
sterilization. Therefore, subsequent chosen methodology for sterilization was
ethanol overnight and ultraviolent lamp 20 min. To elucidate cell behavior on
stent, sterilized parts were placed in non-adherent microplates and seeded with
a final concentration of 40,000 human umbilical vein endothelial cells (HUVEC)
per stent. Endothelial cell proliferation was tested by MTT assay. Results showed
a strong influence of flow rate, number of cells and material over HUVEC growth
(p<0.05), while stent cell geometry did not show significant influence
Biography
Paula Cano Calvo holds a Bachelor’s degree in Biology from
University of Girona. She has successfully completed her final
project entitled 3D Printed PCL and PLA Stents Sterilization
and Culture with Human Endothelial Cells. The project was
performed in research units of University of Girona named
New Therapeutic Targets Laboratory Research Group (Targets
Lab), Oncology Unit in the Department of Medical Sciences
and Product, Process and Production Engineering Research
Group (GREP) in the Department of Mechanical Engineering
and Industrial Construction. She also has performed practices
in TargetsLab where her research was focused on Three
Dimensional Breast Cancer Cell Culture in 3D Matrices and
Mammospheres Generation.
Human endothelial culture cells and sterilization with 3D
printed PCL and PLA stents
Paula Cano Calvo2 , A J Guerra1, M Rabionet 1, 2, T Puig2 and J Ciurana1
1GREP-University of Girona, Spain
2University of Girona, Spain
Paula Cano Calvo et al., Am J Compt Sci Inform Technol 2018 Volume: 6
DOI: 10.21767/2349-3917-C2-005