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American Journal of Computer Science and Information Technology
ISSN: 2349-3917
E u r o s c i c o n C o n f e r e n c e o n
3D Printing and Wireless
Technology
S e p t e m b e r 1 7 - 1 8 , 2 0 1 8
L i s b o n , P o r t u g a l
Wireless and Printing Technology 2018
Cells are traditionally cultured in vitro in two dimensions (2D), but it presents
some limitations, as loss of their in vivo morphology and reduction of cell-
cell and cell-extracellular matrix (ECM) interactions. Hence, three-dimensional
(3D) cell culture models, such as scaffolds, are being developed to overcome the
aforementioned restrictions and to mimic the physiological tissue environment.
Cancer stemcells (CSCs) are a subpopulation of a tumor or cancer cell line, which
are responsible for metastasis and tumor recurrence. They have the capacity
to initiate tumor growth and maintain long term self-renewal, as well as they
exhibit resistance to conventional therapies. Their study in 2D culture is limited
due to the induction of differentiation during CSCs propagation. Interestingly,
3D culture systems can avoid it, besides they can produce an enrichment of
CSCs population. In the present study, scaffolds were manufactured by Fused
Filament Fabrication (FFF) technique with the BCN3D Sigma 3D printer. Five
fabrication parameters were selected to obtain 27 scaffolds designs, which
have different pore size and filament diameter. The tested parameters were
layer height, infill density, infill pattern, infill direction and material flow. Poly
(ε-caprolactone) (PCL) was the material used to manufacture scaffolds. PCL is
commonly used for tissue engineering applications due to its good mechanical
characteristics, as its low melting temperature and low biodegradability, good
biocompatibility, FDA approval and low cost. MDA-MB-231 triple negative breast
cancer (TNBC) cells were cultured on FFF scaffolds to analyze cell efficiency.
Therefore, fabrication parameters of scaffolds with highest cell proliferation
rates were chosen to accommodate 3D cancer cell culture and further analyze
the CSCs enrichment. The enrichment of this malignant subpopulation would
facilitate future experiments to find and develop new therapeutic strategies
against CSCs.
Biography
Emma Polonio Alcalá has completed her Bachelor’s degree in
Biotechnology and Master’s degree in Molecular Biology and
Biomedicine, from University of Girona. Her Bachelors’ degree
project was regarding A New Synthetic Inhibitor of the Fatty
Acid Synthase (Fasn) with Cytotoxic Effects and her Master’s
project was entitled Effects of the New Antitumoral Drug
Abtl0812 in Preclinical Models of Triple Negative Breast Cancer.
She has performed both the projects in New Therapeutic
Targets Laboratory (Targets Lab) research group in the
Department of Medical Sciences from University of Girona and
scored excellent marks. Some of her results were published in
a congress proceeding (Giro- Perafita et al., 2017). Nowadays
her research is focused on The design and fabrication of
biocompatible scaffolds for three-dimensional breast cancer
cell culture, in collaboration with the Product, Process and
Production Engineering Research Group from University of
Girona and is also linked with Targets Lab.
Selection of poly(lactic acid) scaffolds design for triple
negative breast cancer 3d culture
Emma Polonio Alcala1 , M Rabionet1, 2, A J Guerra2 , J Ciurana2
and T Puig1
1University of Girona, Spain
2GREP-University of Girona, Spain
Emma Polonio Alcala et al., Am J Compt Sci Inform Technol 2018 Volume: 6
DOI: 10.21767/2349-3917-C2-005