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Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
E u r o p e a n C o n g r e s s o n
Vaccines & Vaccination
and Gynecologic Oncology
Vaccines & Vaccination and Gynecologic Oncology 2018
O c t o b e r 2 6 - 2 7 , 2 0 1 8
B u d a p e s t , H u n g a r y
Background:
Rabies is a zoonotic viral disease which causes acute encephalitis in humans and animals. The case is most severe in
developing countries where cell culture derived anti-rabies vaccines are unaffordable or the available nervous tissue-derived vaccines
are of questionable immunogenicity and may cause neurological complications. The aim of this study was to adapt local rabies virus
isolates on cell lines and mice brain and to study pathogenicity to intramuscular route of inoculation to develop vaccine strain locally.
Materials & Methods:
The viruses were isolated from rabies dogs’ brain and human saliva and adapted to Swiss albino mice brain and
cell lines (BHK-21 and Vero) by several blind passages to increase viral titer. The viral titers were controlled by titration at each blind pas-
sage both
in vivo
and
in vitro
. For pathogenicity study, mice were inoculated intramuscularly with 250MICLD50/0.1 ml of each adapted
virus isolates and observed for 45 days.
Results:
By titration, a minimum of 10
6.5
TCID50/ml (
in vitro
) and 10
4.5
MICLD50/0.03 ml (
in vivo
) virus titer were obtained. According
to pathogenicity study, only two virus isolates, human origin sululta (HOS) and dog origin (DO) caused 12.5% death.
Conclusion:
Increase in viral titer was significant and it is observed for high viral titer by
in vitro
virus propagation. Death due to intra-
muscular inoculation can indicate the phylogroup origin of the viruses showing decline in virulence due to several blind passages. Ad-
aptation of the viruses to mice brain and cell lines to increase virus infectivity titer significantly affects viral virulence to intramuscular
inoculation. Further, genetic relationship with fixed rabies virus strain need to be studied by molecular techniques and vaccinal strain
should be used from locally isolated viruses.
agagurmu@yahoo.comAdaptation of local rabies virus isolates to high
growth titer and pathogenicity study to develop
vaccinal strain in Ethiopia
Abebe Mengesha Aga
1
, Birhanu Hurisa
1
, Tihitina Tesfaye
1
,
Hailu Lemma
1
, Gashaw G/Wold
1
, Amha Kebede
1
, Tsehaynesh
Mesele
1
, Denis Bankovisky
2
and Kelbessa Urga
1
1
Ethiopian Public Health Institute, Addis Ababa, Ethiopia
2
Pokrov Plant of Biologics, Russian Federation, Russia
Journal of Clinical Immunology and Allergy, Volume: 4
DOI: 10.21767/2471-304X-C2-006
Euro Vaccines 2018