vaccines-gynecologic-oncology-2018-posters

Page 53

Journal of Clinical Immunology and Allergy

ISSN: 2471-304X

E u r o p e a n C o n g r e s s o n

Vaccines & Vaccination

and Gynecologic Oncology

Vaccines & Vaccination and Gynecologic Oncology 2018

O c t o b e r 2 6 - 2 7 , 2 0 1 8

B u d a p e s t , H u n g a r y

An in vitro human cell culture model of NALT to

evaluate humoral immune response to influenza

vaccines

Waleed Mahallawi

1,2

and Qibo Zhang

Taibah University, Saudi Arabia

University of Liverpool, UK

Waleed Mahallawi et al., Journal of Clinical Immunology and Allergy, Volume: 4

DOI: 10.21767/2471-304X-C2-006

Background:

Influenza is a mucosal infection in the respiratory tract which is

transmitted through the nasal mucosa. Human adenoids and tonsils are major

components of local mucosal immune organs namely; nasal-associated lymphoid

tissue (NALT) in humans and are known to be important induction sites for both

mucosal and systemic immunity against upper respiratory tract pathogens. In

this study, an in vitro cell culture model was used to describe the B cell immune

responses induced by influenza vaccines.

Objectives:

Human NALT derived immune cells is an important model to study

immune response. Using NALT to measure immune response against influenza

viruses is mimicking the natural immunity against the viruses.

Results:

Intranasal live attenuated influenza virus-LAIV (FluMist) vaccine

stimulation of NALT mononuclear cells (MNCs) induced IgG, IgA and IgM

antibodies to pH1N1, sH1N1, and sH3N2. Additionally, flu vaccines also induced

mucosal cross-reactive antibodies to aH5N1 following MNCs stimulation.

Conclusion:

It is very important to use the human models to assess pathogens

that causing human health problems such as influenza viruses. This model is very

successful in terms of representing the natural infection.

Euro Vaccines 2018

Biography

He is currently working as a assistant professor in Taibah

University, Saudi Arabia.