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Volume 2
Journal of Environmental Research
Page 73
JOINT EVENT
July 26-27, 2018 Rome, Italy
&
6
th
Edition of International Conference on
Water Pollution & Sewage Management
4
th
International Conference on
Pollution Control & Sustainable Environment
Optimization and degradation of chloroxylenol by free and immobilized
Klebsiella pneumoniae
D2
Ghanem K M
1
, El Ahwany A M D
1
, Farag A M
2
and
Ghanem D A M
1
1
Alexandria University, Egypt
2
National Institute of Oceanography and Fisheries, Egypt
T
wo samples from phenol contaminated soil and waste water were collected for the isolation of bacteria degrading
chloroxylenol. Out of eleven isolates, isolate D2 was the most promising showing a degradation efficiency of 19.9%. The
selected isolate was identified using 16S rDNA analysis as
Klebsiella pneumoniae
D2. Statistical designs were applied to optimize
the medium composition and cultural conditions in favor of increasing the degradation efficiency of
K. pneumoniae
D2. The
Plackett-Burman design was applied to determine the significant factors affecting chloroxylenol degradation. The degradation
efficiency increased to 30.56%. Box-Behnken design was adopted to further investigate the mutual interactions between the
variables and to identify their optimal values that would generate maximum chloroxylenol degradation. Under the optimized
medium composition and culture conditions,
K. pneumoniae
D2 degraded 55.7% chloroxylenol after 24 hrs. Bacterial cells
were adsorbed on different solid supports. The results showed that the degradation efficiency increased up to 88.3% on using 20
cubes of polyurethane foam. The degradation efficiency decreased by 10% on reusing adsorbed cells of
K. pneumoniae
D2 on
polyurethane foam, upto 10 cycles. Immobilization accelerated the degradation. The time was reduced to nine hours reaching
a degradation of 88.3% compared to free cells.
AmaniElAhwany@gmail.comJ Environ Res 2018, Volume: 2