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E u r o s c i c o n C o n f e r e n c e o n
Physical Chemistry and
Analytical Separation Techniques
October 08-09 , 2018
Amsterdam, Nether l ands
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
Physical Chemistry and Analytical Separation Techniques 2018
A
copolymer layer was attached inside a preconditioned 64 cm long silica capillary column with effective length of 56 cm
where internal diameter was 10 µm, after the attachment of 4-chloromehtyphenyl isocayanate and dithiocarbamate initiator
system. Dibutyl tin dichloride catalyst was used for the effective attachment of ligand to silanol group present on inner capillary.
The copolymer immobilized open tubular capillary column resulted in the separation of about 22 peaks (peptides) out of tryptic
digest of cytochrome C sample in capillary electrochromatography with high separation efficiency close to 600,000 plates/column
(990,000 plates/meter) with peak capacity of 220. The use of a mobile phase of high water content was facilitated by the open
tubular nature of the capillary column which make sure the elution of all peptide peaks resulting in relatively short analysis time.
faizy186@gmail.comDemonstration of improved peak capacity in open
tubular capillary column for the separation of
peptides and proteins
Faiz Ali
1
and Won Jo Cheong
2
1
University of Poonch Rawlakot, Pakistan
2
Inha University, South Korea
J Org Inorg Chem 2018 Volume: 4
DOI: 10.21767/2472-1123-C6-018