pharmacognosy-2018-keynote

2 / 5

American Journal of Ethnomedicine
ISSN: 2348-9502
April 16-17, 2018
Amsterdam, Netherlands
Pharmacognosy 2018
Page 16
6th Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Concussive head injury (CHI) is quite common in military
personnel during combat operation for which no suitable
therapy exists till today. In this investigation, we report
that nanodelivery of traditional Chinese medicine, DL-3-n-
butylphthalide (DL-NBP) in CHI that was the most effective in
reducing brain pathology in CHI if given 2 and 4 h after an 8 h
injury or 8 and 12 h after 24 h trauma. Since CHI induces severe
brain ischemia and oxidative stress, in this investigation we also
examined the effects of TiO2-DL-NBP on regional cerebral blood
flow (CBF) and brain pathology in our rat model of CHI. CHI was
inflicted by dropping aweight of 114.6 g on the right parietal skull
bone over a distance of 20 cm in anesthetized rats resulting an
impact of 0.224 N on the skull surface. This impact induces brain
pathology from over 4 h to 24 h. In separate groups of injured
animals, administration of TiO2-nanowired-NBP (40 or 60 mg/kg,
i.p.) 2h and 4 h after injury in 8 h survival group and 8 h and 12 h
after trauma in 24 h survival group. In the untreated and treated
groups, regional CBF (rCBF) in the cerebral cortex, hippocampus,
thalamus, hypothalamus, cerebellum and brainstem was
measured using [125]-Iodine labeled microspheres (15±0.6 µm
o.d.). Untreated CHI resulted in a progressive increase in brain
pathology, reduction in the rCBF (-30 to -50%) and development
of brain edema formation. TiO2-NBP resulted in significant
improvement in rCBF in all brain areas examined. On the other
hand, 80 to 100 mg/kg, dose of normal NBP is needed to induce
comparable c reduction in rCBF and brain pathology in CHI.
These observations show that nanodelivery of NBP has superior
neuroprotective effects in CHI, a feature require additional
investigation for clinical relevance.
Biography
Hari Shanker Sharma is the Director of Research (International Experimen-
tal Central Nervous System Injury & Repair, IECNSIR), University Hospital,
Uppsala University; Professor of Neurobiology (MRC), Docent in Neuro-
anatomy (UU) and is currently affiliated with Department of Surgical Sci-
ences, Division of Anesthesiology and Intensive Care Medicine, Uppsala
University, Sweden. On his research on brain pathology and neuroprotec-
tion in different models, he received prestigious awards from The Laerd-
al Foundation of Acute Medicine, Stavanger, Norway, in 2005 and Distin-
guished International Scientists Collaboration Award (DISCA) by National
Institute on Drug Abuse (NIDA), Baltimore, MD (2006–2008). His current
research is focused on the role of nanoparticles in neurodegeneration and
neuroprotection and nanodrug delivery for novel treatment strategies sup-
ported by European Aerospace Research and Development (EOARD), Lon-
don, UK and US Air Force Research Laboratory, Wright Patterson Air Force
Base, Dayton, OH, USA. He has published over 400 peer reviewed research
papers (H-index 41).
Superior neuroprotective effects of
nanowired delivery of Chinese celery
extract DL-3-n-butylphthalide (DL-NBP) on
concussive head injury
Hari Shanker Sharma
Uppsala University Hospital, Sweden
Hari Shanker Sharma, Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-004