pcos-2018-scientifictracks-abstracts

8 / 11

PCOS 2018 Journal of Clinical and Molecular Endocrinology
ISSN: 2572-5432 Page 24
June 07-08, 2018
London, UK
4th World Congress on
Polycystic Ovarian
Syndrome
Androgen excess is generally considered as one of the major
characteristics of polycystic ovarian syndrome (PCOS).
Evidence from clinical research has revealed increased levels
of oxidative stress (OS) in PCOS patients. Recent research
showed that androgen induced PCOS rat existed over fibrosis,
whichmay have influence on ovary function. Our study aimed to
investigate the possible inhibition of hyperandrogenic ovarian
fibrosis by preventing p66shc-induced oxidative stress. Our
data demonstrated that inhibiting the expression of p66shc
could suppress ovarian oxidative stress, thereby restraining
further fibrosis. In ovarian tissues, reduced fibrosis together
with lowered expression of p66shc and ROS was observed
in resveratrol- and metformin-treated rats. Down-regulation
of fibrogenic factors including collagens, TGF-β, CTGF,
β-catenin and α-SMA as a result of the inhibition of p66shc
was confirmed by western blot, Q-PCR, immunofluorescence
and immunohistochemistry. We also observed that p66shc
was mainly expressed in the nuclei of granulosa cells (GC)
and theca cells (TC). Knockdown of p66shc resulted in
dramatic down-regulation of ROS and fibrogenic factors such
as TGF-β, CTGF, β-catenin and α-SMA in ovarian granulosa
cells and theca cells. Furthermore, inhibition of fibrosis was
accompanied with markedly improved ovarian morphology,
increased luteal cell number and lowered levels of androgen.
These findings suggest that p66shc may be a direct target
of SIRT1 for inducing ROS and thus promoting fibrosis. We
believe that further exploration of the mechanisms of p66shc
in both fibrosis and oxidative stress may provide therapeutic
strategies in improving PCOS symptoms and reproductive
function.
Biography
Yong Wang is a Professor at Medical School, Nanjing University, China
whose research interest focuses on Polycystic Ovary Syndrome. He is dedi-
cated to the teaching of Histology and Embryology.
The critical role of p66shc oxidative stress pathway in
hyperandrogen-induced ovarian fibrosis
Yong Wang and Daojuan Wang
Nanjing University, China
Yong Wang et al., J Clin Mol Endocrinol 2018, Volume 3
DOI: 10.21767/2572-5432-C1-002