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Pain Management 2019 & Internal Medicine 2019
International Journal of Anesthesiology & Pain Medicine
ISSN: 2471-982X
Page 46
JOINT EVENT
7
th
Edition of International Conference on
Pain Management
8
th
Edition of International Conference on
Internal Medicine &
Patient Care
&
March 25-26, 2019
Rome, Italy
Effects of vitamin e and selenium on surgical stress and
lipid peroxidation in dairy cows
Pavol Mudroň
University of Veterinary Medicine and Pharmacy in Kosice, Slovakia
The present trial was aimed to study the effects of vitamin
E and selenium treatment on stress and lipid peroxidation
in dairy cows stressed by omentopexy. Twenty Holstein-
Frisiandairycows,admittedfortreatmentofleftabomasal
displacement,were randomlydivided into twogroups.Ten
hours before surgery 6 g of DL-α-tocopheryl acetate and
67mgofnatriumselenite(Vitaselen®) wereadministered
subcutaneously to 10 cows; the control animals (n=10)
received an equivalent volume of injectable water (40
ml). Abdominal surgery (omentopexy) was performed in
a standing position 16 – 24 hours after admission. Blood
samples were drawn: before vitamin E/Se injection, just
prior to surgery, immediately after surgery, then 15, 30,
60 minutes, and 2, 5, 10, and 24 hours after surgery.
The plasma α-tocopherol was determined by HPLC
using a fluorescent detector. The plasma selenium
levels were determined by fluorometric detection,
plasma TAC was analysed spectrophotometrically, and
TBARS spectrofluorometrically. The serum cortisol was
determined by chemiluminiscent enzyme immunoassay.
Statistical analysis was carried out by a two-factorial
analysis of variance (one repeated factor: time, one
grouping factor: treatment). The injection of vitamin E
and selenium produced a rapid rise (p<0.05) in blood
α-tocopherol and selenium concentrations. Two-way
ANOVA did not show significant treatment effect on
plasma TBARS and TAC in our trial. In contrast, a certain
effect of the treatment could be found on serum glucose
and WBC count (p<0.05). Serum cortisol concentrations
increased in both groups after surgery (p<0.05) and two-
way ANOVA revealed significant effect of treatment on
cortisol levels (p<0.05). In conclusion, the administration
of Vitamin E and selenium resulted in weaker cortisol
response in experimental animals, however, no significant
effects of a single vitamin E/Se injection on blood TBARS
were found.
Biography
Pavol Mudroň graduated as a DVM at the University of Veteri-
nary Medicine (Kosice, Slovakia) in 1985. In 1996 successfully
defended his PhD thesis on “Role of vitamin E in immune re-
sponse” In 1998 he became Associated Professor and in 2006
Diplomate of the European College of Bovine Health Manage-
ment. At the moment he is full professor and head of the Clin-
ic of Ruminants at the University of Veterinary Medicine and
Pharmacy in Kosice.
Pavol
,mudron@uvlf.skPavol Mudroň, Int J Anesth Pain Med 2019, Volume 5
DOI: 10.21767/2471-982X-C1-006