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Pain Management 2019 & Internal Medicine 2019
International Journal of Anesthesiology & Pain Medicine
ISSN: 2471-982X
Page 53
JOINT EVENT
7
th
Edition of International Conference on
Pain Management
8
th
Edition of International Conference on
Internal Medicine &
Patient Care
&
March 25-26, 2019
Rome, Italy
Tarek A Alshazly et al., Int J Anesth Pain Med 2019, Volume 5
DOI: 10.21767/2471-982X-C1-006
Molecular identification of Methicillin resistant
Staphylococcus aureus and evaluation of Panton Valentine
Leukocidin Gene as a sole marker for CA-MRSA
Tarek A Alshazly
3
, Sahar M Ali
1
and
Eman AM Bayoumi
2
1
Menufiya University
2
Ain Shams University, Egypt
3
Mansoura University, Egypt
Background
: Infections caused by MRSA represent
a growing problem and a challenge for healthcare
institutions.
Aim of work
: To compare the efficacy of phenotypic
methods for identification of MRSA with genotypic
method and to evaluate if PVL gene could be used as sole
marker for CA-MRSA.
Material & methods
: 88 isolates of Methicillin-resistant
Staphylococcus aureus (MRSA) were included in this
study. Phenotypic identification was done by Oxacillin DD,
Penicillin-Binding Protein 2a (PBP2a), latex agglutination
and Cefoxitin DD. Molecular detection of mec-A gene and
PVL gene was done by real time PCR.
Results
: The PVL genewas detected among 46.8%of CA-
MRSA, while only in 12.2% Hospital-associated MRSA
(HA-MRSA). 88 mec A positive isolates were identified
as MRSA by oxacillin DD, PBP2a latex agglutination and
cefoxitin disc diffusion (DD) methods with sensitivity
of 92%, 98.8% and 100% respectively. Patients with
Panton-Valentine leukocidin (PVL) positive CA-MRSA
were significantly younger males mostly of skin and
soft tissues origin (p = 0.002). It showed distinctive
antibiogram profile being significantly more sensitive to
Levofloxacin, Moxifloxacin, Ciprofloxacin, Gentamicin,
Clindamycin (p<0.001) and Tetracycline (p <0.05).
Conclusion
: The best phenotypic method for detection of
MRSA is the combination of the cefoxitin DD and the latex
agglutination test. The presence of PVL gene cannot be
used as a sole marker for CA-MRSA. [Sahar M Ali, Eman
AM Bayoumi, Tarek A Alshazly. Molecular Identification
of Methicillin resistant Staphylococcus aureus and
Evaluation of Panton-Valentine Leukocidin Gene as a sole
marker for CA- MRSA.
Biography
Dr. Tarek A. Elshazly has completed his MD from Mansoura
University Egypt. He is assistant professor in Internal Medicine
Department with different activities which include teaching ac-
tivities for both under and post graduates, managing inpatient
and outpatient clinic, supervising endoscopic unit in special-
izedmedical hospital and emergency hospital. He is interested
in research activities and publication.
drtarekameen@yahoo.com