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Pain Management 2019 & Internal Medicine 2019
International Journal of Anesthesiology & Pain Medicine
ISSN: 2471-982X
Page 77
JOINT EVENT
7
th
Edition of International Conference on
Pain Management
8
th
Edition of International Conference on
Internal Medicine &
Patient Care
&
March 25-26, 2019
Rome, Italy
Int J Anesth Pain Med 2019, Volume 5
DOI: 10.21767/2471-982X-C1-006
Evaluation and comparison of stability and reliability of CBC
parameters determined by using automatic Celltac G MEK-
9100 hematology analyzer during extended storage at 4°c
Sajid Hussain
Multan Institute of Nuclear Medicine and Oncology - Nishtrar Medical University, Pakistan
Introduction:
Sample stability is necessary for the
maintenance of the quality for the final results obtained
at different storage time intervals during CBC analysis.
In our present study, we have evaluated the stability of
various CBC parameters of blood samples stored in k2-
EDTA (BD) vials at 4°C (extended storage time: 10 days).
Materials & Methods:
Blood sample (2.5 ml) was drawn
directly in K2-EDTA vials. Measurements were done
by using MEK-9100 hematology analyzer at regular
intervals over an extended period up to 246 h (10 days).
Significant differences were analyzed by paired student’s
t-test. Mean percent differences of the all intervals were
compared with baseline means.
Results:
Among CBC parameters, WBC count was stable
for up to 126 h, RBC and HGB levels were statistically
stable for up to 186 h and 90 h. No significant changes
were observed in NE, LY, MO, EO and BA for up to 42 h,
42 h, 66 h, 66 h, and 6 h respectively. PLT counts were
stable for 6 h. Furthermore, results of HCT, MCV, MCH,
MCHC, RDW-CV, RDW-S, PCT and MPV were statistically
stable for upto 54 h, 42 h, 18 h, 30 h, 42 h, 30 h, 6 h and 6
h respectively.
Conclusion:
Estimation of RBC, WBC and HGB were
qualitatively reliable ~186 h, 126 h and 90 h respectively.
However, most parameters of CBC were unchanged
~48 h except for the PLT (6 h). To avoid changes in few
parameters, such as MPV, basophiles, it is best to store
the sample at 4°C if any delay is anticipated.
sajid9596@gmail.com