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Pain Management 2019 & Internal Medicine 2019
International Journal of Anesthesiology & Pain Medicine
ISSN: 2471-982X
Page 61
JOINT EVENT
7
th
Edition of International Conference on
Pain Management
8
th
Edition of International Conference on
Internal Medicine &
Patient Care
&
March 25-26, 2019
Rome, Italy
Int J Anesth Pain Med 2019, Volume 5
DOI: 10.21767/2471-982X-C1-006
The comparison effect between Bangun-Bangun leaves
(Coleus Amboinicus) ethanol extracts and carrots (Daucus
Carota) juice as analgetic on mice (Mus Musculus) induced
by acetic acid
Yunita Sari Pane, Sufitni Hasan, Yetty Machrina, Nenni Dwi Aprianti Lubis and
Dina Keumala Sari
Universitas Sumatera Utara, Indonesia
T
he development of herbal medicine in Indonesia
is growing rapidly. The government support many
universities for doing some researches about herbal. The
aim of this study to compare effect between Bangun-
bangun leaves ethanol extract (EDBB) and Carrots juice
(CJ) as analgetic on mice that induced by acetic acid.
The experimental animals used were 24 mice divided
into 4 groups, ie group-I (negative control/placebo given
aquadest 0.2 cc/20 g BWmice); group-II (positive control,
paracetamol (PCT) with dose of 2 mg/20 g BW mice);
group-III (EDBB) ethanol extract with dose of 144 mg/20
g BWmice); and group-IV (CJ) with dose of 5mg/20 g BW
mice). All of these treatments were administered orally
10 minutes before the induction of acetic acid 1% (0.3
cc/20 g BW mice) intraperitoneally. Observations were
made by looking at the writhing response (observed for
1 hour) and the amount of infiltration of leukocyte cells at
the injection site. Permanent cervical fracture execution
was performed at the end of the study (4 hours after
induction of acetic acid) to see themigration of leukocyte
to the peritoneal tissue and examined histopathologically
by the light microscope Olympus 400x magnification
field. The results were analyzed using SPSS and ANOVA
then post hoc Tukey analysis. In the previous study, Pane
et al. (2018) get an effective dose of reducing the pain
of 144 mg/20 gBW mice EDBB. Carvacrol contained in
the Bangun-bangun (Coleus amboinicus) leaves was
suggestedhadefficacyasananalgesicagentbyinhibition
of peripheral mediators that could be related to its strong
antioxidant effects observed in vitro suppressed hyper
nociceptive and inflammation pathways. In addition,
Carvacrol has anti-inflammatory effects by reducing the
production of inflammatory mediators, such as IL-1β and
prostanoid, possibly via the induction of IL-10 release.
The effect of anti-inflammatory cytokines contribute
to controlling the central and peripheral effects of pain
stimulation may contribute to decreased stimulation
of nociceptive pathways, facilitating pain control.
Meanwhile, Carrot (Daucus carota) is also known to work
as an external oxidant which is believed to be efficacious
as an analgesic with chemistry stimulation on mice. It
suggested that the carrot contained beta carotene as an
analgesic to inhibit free radical. The present study proved
that the decrease of excitatory pain in all treatment
groups (I, II, III and IV) was significantly different,
whereas p=0.000. The comparison of mean values ±
SEM decreased excitatory pain group I-II (255.00±22.22;
88,33±14,58, p=0.000. Group I-III (255.00±22.22; 50,83