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J Obes Eat Disord, 2017
ISSN: 2471-8203
August 23-24, 2017 | Toronto, Canada
allied
academies
INTERNATIONAL OBESITY, BARIATRIC AND
METABOLIC SURGERY SUMMIT AND EXPO
O
besity and overweight are important risk factors for
diseases, includingmany forms of cancer. It is known that
damage of the genetic material plays a key role in malignant
transformation, therefore the aim of present study was to
investigate the induction of DNA damage by obesity and its
prevention by gallic acid (GA), a common dietary phenolic in
various inner organs of mice. The extent of DNA damage was
monitored in female and male C57BL/J mice which were fed
either with a standard diet or a high fat diet for 15 weeks.
Furthermore the obese animals received GA (2, 25, 50 mg/kg
B.W) in drinking water. We observed significant induction of
DNA damage in single cell gel electrophoresis (SCGE) assays
which detect double strand breaks, single strand breaks
and apurinic sites, in liver, brain, colon and blood, as well
as increased formation of oxidized purines/pyrimidine, in
obese males and females, while only moderate effects were
observed in white adipose tissue. Low doses of GA which
correlated to the daily intake in humans led to pronounced
reduction of DNA damage in all organs. Biochemical
measurements showed that the pattern of DNA damage
correlates with changes of glucose metabolism but not with
the activation of NF-kappa B in hepatic tissue and also not
with alterations of the redox status (GSH, TBARS and GPx).
Taken together, our findings indicate that obesity causes
genomic instability as well as oxidative damage of DNA-
bases in multiple organs of both sexes which may be related
to alterations of the glucose metabolism. Furthermore,
our results show that these effects can be reduced by low
doses of GA which is contained in certain plant foods, and
beverages.
e:
n1249216@student.meduniwien.ac.atImpact of gallic acid on DNA damage and health related biochemical parameters in obese mice
Tahereh Setayesh, Franziska Ferk, Armen Nersesyan, Miroslav Mišík, Wolfgang Huber, Elisabeth Lang, Micheal Grusch, Elisabeth Haslinger, Alexander
Haslberger and Siegried Knasmüller
Medical University Vienna, Austria
J Obes Eat Disord, 3:2
DOI: 10.21767/2471-8203-C1-003