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Volume 4, Issue 2
American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3
rd
World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12
th
WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Anti-inflammatory activity of
Euphorbia tithymaliodes
L. ethanol extract in lipopolysaccharide-
stimulated RAW264.7 macrophages
Seong Gu Hwang
1
, Theresia Galuh Wandita
1
, Seong Hyeon Bae
1
and
Joseph Dela Cruz
2
1
Hankyong National University, South Korea
2
University of the Philippines Los Banos, Philippines
E
uphorbia tithymaloides
L. is native plant growing in tropical and subtropical areas of Asian countries. It has been known as
traditional medicine with a wide range of healing properties such as anti-oxidant, anti-inflammatory, anti-hemorrhage, anti-
diabetic, antibiotic, and anti-tumoral. It contains some bio-active compounds such as beta-sitosterol, cycloartenone, octacosanol,
oxime, etc. In earlier
in-vitro
study, octacosanol has reduced the expression of mRNA or protein of pro-inflammatory cytokines.
This study is aimed to evaluate the anti-inflammatory activity of
Euphorbia tithymaloides
L. ethanol extract (ETE) in RAW264.7
macrophages. Anti-inflammatory activity was studied by treating RAW264.7 murine macrophages cells with increasing concentration
of the ETE extract (50, 100, 200, and 400 µg/ml). Lipopolysaccharide (LPS) was used to activate the cells. CCK-8 assay and Griess
reagent were used to examine the cell viability and Nitric Oxide (NO) production, respectively. The result shows increasing
concentration by 200 µg/ml increases cell proliferation then declines. On the other hand, Nitric Oxide (NO) production decreases
with the increase of concentration of the extract. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) was used to measure
the mRNA expression of pro-inflammatory cytokines. Western blotting was also done to examine the effect of ETE extract on the
protein expression of RAW264.7 murine macrophages cells. It has been shown that increasing concentration decreased the mRNA
expression of IL-6, iNOS, COX-2, TNF α, IFN γand NF-kB, as well as protein expression. In conclusion, the results support the
empirical use of
Euphorbia tithymaloides
L. ethanol extract as an anti-inflammatory medicine.
Biography
Seong Gu Hwang has completed his higher qualification from Hankyong National University, Korea and currently working in Department of Animal Life and
Environmental Science, Hankyong National University, Korea.
sghwang@hknu.ac.krSeong Gu Hwang et al., American Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-002