2 / 35
Infectious Diseases 2018
Journal of Prevention and Infection Control
ISSN: 2471-9668
Page 40
June 07-08, 2018
London, UK
8
th
Edition of International Conference on
Infectious Diseases
Background:
Hospital acquired infections (HAI)/nosocomial
infections affect >20% of patients in intensive care units and
have a high associated mortality rate of >30%. Patients receiving
neurosurgical intensive care are exposed to several risk factors
like multiple trauma, altered consciousness, impaired protective
reflexes etc., for acquisition of nosocomial infections which can
be presented as pneumonia, urinary tract infection, meningitis etc.
Nosocomial meningitis is mainly seen in neurosurgical patients.
Due to anatomical restrictions, the inflammatory response to
intracranial bacterial infections exposes swollen brain tissues
to pressure and ischemia, in a life-threatening condition. Rapid
diagnosis and immediate empirical antibiotic therapy is highly
important. However, diagnosing meningitis in patients after
neurosurgical procedures is complicated, due to brain tissue
damage and changes in cerebrospinal fluid (CSF) caused by
surgery. Moreover, altered consciousness can make it difficult to
establish a diagnosis in patients on ventilators that develop fever
after neurosurgical operations. Neutrophils are importantmembers
of innate immunity that are activated by microbes. Neutrophils can
kill pathogens extracellularly by releasing neutrophil extracellular
traps (NETs) that are composed of chromatin bound to selected
cytoplasmic proteins.
Materials & Methods:
Chromatin has a high affinity to aniline dyes.
Based on metachromatic staining’s principle, we prepared simple
and rapid tests that detected presence of NETs by immediate
interaction to chromatin in CSF. CSF from neurosurgical patients
that developed fever post-operatively were analyzed with the
test and the results were compared with conventional diagnostic
methods.
Results:
CSF samples (n=163) were collected consecutively from
patients. A positive CSF culture was chosen as golden standard.
The results showed that the test detected culture positive bacterial
meningitis with 100% sensitivity, 74.7% specificity and negative
predictive value 100%.
Conclusion:
The rapid test might be a valuable tool to detect
bacterial meningitis immediately after lumbar puncture in patients
with suspected infection
Biography
Amir Ramezani studied medicine at Karolinska institute Sweden 1988-1993,
recieved his Medical degree. 1993. He has done his residency in neurosurgery
in Linköping university hospital and become specialist in neurusurgery 2004.
Work at the moment as consultant neurosurgeon and head of spinal unit in
Linköping university hospital in Sweden and PhD student at Linköping univer-
sity since 2016.
amirramezani@icloud.comRapid bedside diagnosis of bacterial meningitis
Amir Ramezani
1
, Lars Eng
2
, Annette Theodorsson
1
and
Fariba Nayeri
2
1
Linköping University, Sweden
2
PEAS Institute, Sweden
Amir Ramezani et al., J Prev Infect Cntrol 2018, Volume 4
DOI: 10.21767/2471-9668-C1-003