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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 111
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
R
ecent research explained that there may be various causes of rheumatoid arthritis i.e. an autoimmune disorder. Different genes
including
HLA, TRF
(receptor family) may be involved in the occurrence of rheumatoid arthritis. ACE gene polymorphisms are
also considered to be associated with rheumatoid arthritis. The main focus of this study was to check the association of ACE
gene I/D polymorphism with rheumatoid arthritis. The general population of Pakistan was selected. Total numbers of the blood
samples were 80, out of which 40 were of rheumatoid arthritis patients and 40 were of healthy controls. Body weight, blood
glucose levels, rheumatoid factor and gender were the parameters noted in the performa. The mean age of male patients was
51.833±0.17 and that of female patients were 54.90±1.65. The angiotensin enzymes cleave the proteins and regulate fluids and
salts balance in the body. The blood samples were collected and stored at 4∏C for further purposes. Serum samples were also
collected for screening of the blood samples. Other immunoassays used were agglutination assays including C-reactive protein
levels in blood and rheumatoid factor of the patients. Immunofluorescence of the rheumatoid positive samples was also done.
The mostly observed patterns for anti-nuclear antibodies were homogenous and speckled. Blood glucose levels of the patients
were recorded. In the study, the ACE gene was selected for the study of ACE gene insertion or deletion polymorphism. Nested
PCR cycle was run for the specified sequence portion of ACE gene intron 16. All the controls showed 100% DD genotype. The
22.5% subject samples showed the ID genotype and 77.5% showed the DD genotype. No subject either control or patient showed
II genotype. As a result, the null hypothesis was rejected and p value was less than 0.05. A significant association was found
between rheumatoid arthritis and ACE gene I/D polymorphism
Amnas616@gmail.comACE gene insertion/deletion polymorphism in
Pakistani rheumatoid arthritis patients
Sajid A and Hussain N
University of the Punjab, Pakistan
Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003