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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 109
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
Background:
Monocytes are important effector cells during
Leishmania
infection and changes in their functions may impact
development of immunity. However, functional characteristics of monocytes in visceral leishmaniasis (VL) patients remains
poorly understood.
Methods:
Peripheral blood monocytes from VL patients and healthy endemic controls (ECs) from Muzaffarpur, Bihar, India were
isolated and compared
ex-vivo
using cell-culture techniques, flow cytometry and RT-qPCR.
Results:
A blood monocyte population with a gene signature comprising upregulated expression of
TGM2, CTLRs, VDR, PKM,
SOCS1, CAMP1
, accompanied by downregulated expression of
NOS2
and
HIF1A
was observed in VL patients, compared to ECs. VL
patient monocytes also had impaired expression of chemokine receptors, adhesion molecules and decreased frequencies of IL-
1β- and IL-6-producing cells. Importantly, monocytes from VL patients had a marked reduction in their capacity for phagocytosis
of amastigotes, as well as expression of p47phox, p67phox and ROS-production.
Conclusions:
VL monocytes express anti-inflammatory molecules and lack a classically activated phenotype. They have reduced
expression of molecules related to activation and anti-parasitic effector functions, indicating that monocytes are skewed towards
anti-inflammatory phenotype. These findings provide insights into functional status of monocytes during VL and advise that
therapeutic manipulation of this important cell population may result in favourable patient outcomes
neetu24sep@gmail.comPeripheral blood monocytes with an anti-
inflammatory phenotype display limited
phagocytosis and oxidative burst in visceral
leishmaniasis patients
Neetu Singh
1
, Rajiv Kumar
2
, Shashi Bhushan Chauhan
1
, Christian
Engwerda
3
and Shyam Sundar
1
1
Institute of Medical Sciences, Banaras Hindu University, India
2
Banaras Hindu University, India
3
QIMR Berghofer Medical Research Institute, Australia
Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003