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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 107
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
H
ost inflammatory immune response has been described as an essential step of bone healing process. In this context,
immunoregulatory molecules that consequently modulate inflammatory cell migration, and the subsequent bone repair,
have been considered as potential targets for improving bone repair. This study aim evaluates the experimental role of the
immunoregulatory molecule VIP in the process of alveolar bone healing in C57Bl/6 (WT) mice. Experimental groups (N=5/
time point) comprised animals submitted to right upper incisor extraction, maintained how control or treated with VIP (Sigma
Aldrich-0.05 mg/kg, IP, 24/24h) or VIP-Antagonist (VIP-Antagonist-GRF1-29-Sigma Aldrich-0.05 mg/kg, IP, 24/24h) starting
one day before surgery; assessed by microtomographic-μCT and histomorphometric analysis, in the periods 0h,3,7 and 14d,
for quantification of tissue repair indicators and cell migration to the repair site. The results of μCT did not show significant
difference between groups in relation to hyperdense regions. The histomorphometric analysis demonstrated a greater area of
newly formed bone tissue and a higher number of osteoblasts in the VIP-antagonist group when compared to the control and
VIP groups (7d). Referent to osteoclasts density, the groups VIP and VIP-Antagonist presents a major density in relationship
to the control groups (14d), which suggests a greater bone remodelling in these groups. Connective tissue formation was also
analysed, with the density similar of collagen fibers and blood vessels between groups. Density of fibroblasts, the group treated
with VIP-antagonist show a significant diminution compared to the control group (7d). Density of inflammatory infiltrate was also
observed, and the group treated with VIP-antagonist showed a higher density in relation to the VIP and control groups. Density
of blood clot post-extraction, the group control presents a major density when compared with the VIP-antagonist. These results
suggest that inhibition of VIP modifies the course of post-exodontic alveolar bone repair; and further analyses are underway to
determine the immunoregulatory mechanisms involved in this modulation.
michelle_soriani@hotmail.comModulatory effects of VIP (vasoactive intestinal
peptide) in the bone healing process
Michelle de Campos Soriani Azevedo
1
, Priscila Maria Colavite¹,
Carolina Favaro Franciscone¹, Angelica Cristina Fonseca¹, Jessi-
ca Lima Melchiades¹, André Petenucci Tabanez¹, Ana Paula Fa-
varo Trombone² and Gustavo Pompermaier Garlet¹
1
University of São Paulo, School of Dentistry of Bauru (FOB/USP), Brazil
2
University of the Sacred Heart (USC), Bauru, Brazil
Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003