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Laser Optics & Photonics and Atomic & Plasma Science 2018
J u l y 1 6 - 1 7 , 2 0 1 8
P r a g u e , C z e c h R e p u b l i c
Page 64
American Journal of Computer Science and Information Technology
ISSN: 2349-3917
E u r o S c i C o n J o i n t E v e n t o n
Laser Optics & Photonics and
Atomic & Plasma Science
Katel Herve-Aubert et al., Am J Compt Sci Inform Technol 2018, Volume 6
DOI: 10.21767/2349-3917-C1-003
R
esearch in nanomedicine is receiving increasing attention since the
beginning of the twenty-first century. There is a hope that unique properties
of nanosystems (NS) may help to improve diagnosis and therapy of diseases.
Nanosystemsof different design (quantumdots, liposomes, dendrimers, carbon
nanotubes, microbubbles, metallic nanoparticles…) were proposed for their
use as imaging, therapy or theranostic (therapeutic plus diagnostic) agents.
The development of these nanosystems in medicine requires investigating
their biodistribution in cells and tissues. For this purpose, a common strategy
consists of labelling the nanosystems with a fluorescent dye. However, such
a labelling does not always allow a reliable tracking of nanosystems, namely
due to: i) the degradation and/or the quenching of fluorophores by interaction
with the biological environment; ii) the release of fluorophores from the NS,
which prevents to know if the observed fluorescence does correspond to
the nanosystem. To circumvent these limitations, we developed a rational
NS design and used spectral analysis of the NS fluorescence in solution
and in cells. Rationally designed NS were composed of an inorganic core
(superparamagnetic iron oxide nanoparticles – SPIONs or gold nanoparticles)
coated with an organic shell made of molecules covalently attached to the
core (fluorophores and polyethylene glycol, PEG
5000
). Thus, the fluorescent
labels were hidden under the PEG
5000
layer. This was intended to (i) protect
the fluorophores from quenching and degradation, (ii) reduce the risk of their
release from the nanoparticles, and (iii) avoid the possible effect of these
labels on the nanoparticles surface properties, which are critical for their
stability and biological interactions. Labelling was optimized by varying the
dye concentration and due to the purification steps. For the more relevant
optical assessment of these optimized nanosystems within cancer cells, their
fluorescence has been analyzed both by spectroscopy and confocal spectral
imaging.
Biography
Katel Herve-Aubert has completed her PhD from University
of Rennes 1, France in 2005. She is an Associate Professor
in Nanomedicine and Nanoprobes laboratory (EA6295) at the
University of Tours since 2006. This interdisciplinary team de-
velops bio-analytical methods and nanomedicine technology
for drug delivery and disease diagnostics. Her speciality is the
Synthesis and Characterization of Nanomedicines.
katel.herve@univ-tours.frOptimized Synthesis And Assessment Of Fluorescent Dye-
Labelled Nanosystems
Katel Herve-Aubert
1
, Emilie Allard-Vannier
1
, Anastasia
Ignatova
2
, Alexey Feofanov
2, 3
and Igor Chourpa
1
1
University of Tours, France
2
Shemyakin-Ovchinnikov IBCh-RAS, Russia
3
Lomonosov Moscow State University, Russia