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Notes:
Volume 4
Trends in Green Chemistry
ISSN: 2471-9889
Page 96
JOINT EVENT
Environmental Chemistry 2018 &
Green Technologies 2018
September 20-22, 2018
September 20-22, 2018 Berlin, Germany
&
7
th
Edition of International Conference on
Green Energy, Green Engineering and Technology
8
th
International Conference on
Environmental Chemistry and Engineering
Evaluation of novel thermo tolerant haloalkaliphilic bacterium
Halomonas stevensii
for biomitigation of
gaseous phase CO
2
: Energy assessment and product evaluation studies
Somesh Mishra
Indian Institute of Technology Delhi, India
P
resent work deals with the bio-mitigation potential of gaseous phase CO
2
by chemolithotrophic bacterium
Halomonas
stevensii
isolated from haloalkaliphilic habitat using thiosulfate ion (S
2
O32-) as an energy source.
H. stevensii
was tested
for various abiotic stress tolerance such as salt [1 - 14% (%w: v)], temperature (35-80oC) and pH (4-12). Batch studies were
conducted for 6 days at 15 (±1) % (% v:v) inlet CO
2
concentration to find the CO2 fixing capability of
H. stevensii
under varying
concentration of energy substrate i.e. 0, 50 and 100 mm Na
2
S
2
O
3
. Approximately 98% CO
2
removal from gaseous phase was
achieved at 50 and 100 mm Na
2
S
2
O
3
. Biomass productivity was estimated in terms of maximum biomass productivity (P-Max)
and specific growth rate (µMax). 6th day sample (biomass and supernatant) obtained from 100 mm Na
2
S
2
O
3
batch study
was characterized by FTIR and GC-MS to identify the products formed from CO
2
fixation. The evaluation of CO
2
fixation
by
H. stevensii
into primary metabolite was carried out by growing the
H. stevensii
at 5%, 10% and 15 % (% v: v) inlet CO
2
concentration for the duration of 6 days. The obtained leachate was further analyzed by GC for the quantification of fatty
alcohols. The utilization of gaseous phase CO
2
by
H. stevensii
is also proven by conducting the approximate materials balance
and energy assessment for the present CO
2
fixation process.
somesh.mishra@pilani.bits-pilani.ac.inTrends in Green chem 2018, Volume 4
DOI: 10.21767/2471-9889-C2-012