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Dental Treatment 2018
Dentistry and Craniofacial Research
ISSN: 2576-392X
Page 55
September 10-11, 2018
Zurich, Switzerland
25
th
International Conference on
Dental Treatment
Objective:
The aim of this study was to assess the effect of using
enamel matrix proteins (EMP) or dentin collagen in the treatment
of human root surfaces as bio-modifiers.
Material & Methods:
Thirty root slices were obtained from
proximal surfaces of fifteen lower anterior teeth that had been
extracted due to hopeless periodontitis. The root slices were
randomly divided into three groups and subjected to one of the
following treatments: I) Control group (no treatment received),
II) Enamel matrix proteins, III) Dentin collagen. Periodontal cells
were seeded in each group with a concentration of (5x10
3
/20 µl),
and incubated for seven days on dentin surfaces of the root slices.
Following incubation, the attached cells were calculated and also
the cell morphologies were evaluated by SEM. The statistical
analysis was done using One-way ANOVA test at (p≤0.05).
Results:
Second and third groups did not show significant
differences regarding attached cells (p>0.05), but showed
significance in comparison to control. However, the attached
cells were highest in second group (EMP) and lowest in the
control group. Regarding cell morphologies, control group had
mostly round to oval cells whereas other two experimental groups
showed evidently flat elongated cells.
Conclusion:
Despite EMP showed the highest gained attached
cells, both of EMP and dentin collagen showed comparable
effective positive way on the adhesion and attachment of cells to
root surfaces. This may has a critical major role in the periodontal
regenerative treatment.
mahmoud_672000@yahoo.comA regenerative treatment following root surface
biomodification using enamel matrix proteins or dentin
collagen – cell culture experimental process
Mahmoud Helmy Belal
Damanhour University, Egypt
J Dent Craniofac Res 2018, Volume 3
DOI: 10.21767/2576-392X-C3-009